NR1D1

Gene Symbol NR1D1
Entrez Gene 9572
Alt Symbol EAR1, THRA1, THRAL, ear-1, hRev
Species Human
Gene Type protein-coding
Description nuclear receptor subfamily 1, group D, member 1
Other Description Rev-ErbAalpha|V-erbA-related protein 1|nuclear receptor Rev-ErbA-alpha|nuclear receptor subfamily 1 group D member 1|rev-erbA-alpha
Swissprots Q0P5Z4 Q15304 P20393
Accessions AAS48347 EAW60636 P20393 AK300155 BAG61939 AK315270 AY429554 BC047875 AAH47875 BC056148 AAH56148 BM671861 HQ692861 ADZ17372 M24898 AAA52335 M34339 AAA36561 M34340 AAA36562 X72631 CAB53540 NM_021724 NP_068370
Function Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARTNL/BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE),
Subcellular Location Nucleus. Cytoplasm {ECO:0000250}. Cell projection, dendrite {ECO:0000250}. Cell projection, dendritic spine {ECO:0000250}. Note=Localizes to the cytoplasm, dendrites and dendritic spine in the presence of OPHN1. {ECO:0000250}.
Tissue Specificity Widely expressed. Expressed at high levels in the liver, adipose tissue, skeletal muscle and brain. Also expressed in endothelial cells (ECs), vascular smooth muscle cells (VSMCs) and macrophages. Expression oscillates diurnally in the suprachiasmatic nucleus (SCN) of the hypothalamus as well as in peripheral tissues. Expression increases during the differentiation of pre-adipocytes into mature adipocytes. Expressed at high levels in some squamous carcinoma cell lines. {ECO:0000269|PubMed:2539258}.
Top Pathways Circadian rhythm

Phospho-Rev-erbα (Ser55/59) Antibody - 2129 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, either untransfected or transfected with DYKDDDDK-tagged Rev-erbα expression constructs, using Phospho-Rev-erbα (Ser55/Ser59) Antibody (upper) or DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody) #2368 (lower). The phospho-specificity of the antibody was verified by transfecting cells with a mutant DYKDDDDK-Rev-erbα (Ser55/59Ala) expression construct and by incubating 293/Rev-erbα (WT) extracts with (+) or without (-) λ phosphatase.

Rev-Erbα (E1Y6D) Rabbit mAb - 13418 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1Y6D
Western blot analysis of extracts from various cell lines and tissues using Rev-Erbα (E1Y6D) Rabbit mAb.

Rev-erbα Antibody - 2124 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Rev-erbα (E-12) - sc-393215 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rev-erbα (E-12): sc-393215. Western blot analysis of Rev-erbα expression in non-transfected: sc-117752 (A) and mouse Rev-erbα transfected: sc-125901 (B) 293T whole cell lysates.

Rev-erbα (K-12) - sc-47624 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rev-erbα (K-12): sc-47624. Western blot analysis of Rev-erbα expression in Hep G2 (A) and T-47D (B) whole cell lysates.

Rev-erbα (H-49) - sc-135241 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Rev-erbα (N-19) - sc-47626 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Rev-erbα (N-15) - sc-47625 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rev-erbα (N-15): sc-47625. Western blot analysis of Rev-erbα expression in mouse adipose tissue extract.

Rev-erbα (RS-14) - sc-100910 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Rev-erbα (RS-14): sc-100910. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear localization.

Anti-NR1D1 antibody - ab90005 from Abcam

Prices $376.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-fixed, formalin-embedded fetal kidney showing nuclear staining with ab90005 at a dilution of 1/100: (A) without immunizing peptide, (B) blocking with immunizing peptide

Anti-NR1D1 antibody - ab56754 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
NR1D1 antibody (ab56754) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.

Anti-NR1D1 antibody [4F6] - ab115552 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4F6
Overlay histogram showing HeLa cells stained with ab115552 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115552, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-NR1D1 antibody [A8740] - ab41939 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone A8740

Anti-NR1D1 antibody [EPR10376] - ab174309 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10376
Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling NR1D1 with ab174309 at 1/50 dilution.

Anti-NR1D1 Antibody (aa290-339) IHC-plus™ - LS-B8100 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NR1D1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8100 dilution 5-10 ug/ml.

Anti-NR1D1 Antibody (Internal) IHC-plus™ - LS-A6006 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NR1D1 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-NR1D1 Antibody (clone 4F6) IHC-plus™ - LS-B4487 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4F6
Anti-NR1D1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4487 concentration 5 ug/ml.

Anti-NR1D1 Antibody (N-Terminus) - LS-C126066 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NR1D1 Antibody (aa191-219, AP) - LS-C216032 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NR1D1 Antibody (aa191-219, Biotin) - LS-C216034 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NR1D1 Antibody (aa191-219, APC) - LS-C216033 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NR1D1 Antibody (aa191-219, Azide-free, HRP) - LS-C216035 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NR1D1 Antibody (aa190-219) - LS-C323429 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NR1D1 Antibody (aa191-219, FITC) - LS-C216036 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NR1D1 Antibody (aa38-87) - LS-C80071 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.
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