Anti-Nrf2 (phospho S40) antibody [EP1809Y]

• Supplier: Abcam
• Catalog: ab76026
• Prices: $404.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1809Y
• Applications: ICC/IF ICC/IF IHC-P WB IP ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic phospho-peptide (Human) corresponding to residues near serine 40 of Nrf2
• Blocking Peptide: Nrf2 (phospho S40) peptide
• Description: Rabbit Monoclonal
• Gene: NFE2L2
• Conjugate: Unconjugated

Product images

Overlay histogram showing Jurkat cells stained with ab76026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76026, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] (ab76026) at 1/20000 dilutionLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysate treated with APLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76026 at 1/100 dilution.

ab76026 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.See Abreview

ICC/IF image of ab76026 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76026, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab76026 showing positive staining in Breast carcinoma tissue.

ab76026 showing positive staining in Cervical carcinoma tissue.

ab76026 showing positive staining in Ovarian carcinoma tissue.

ab76026 showing positive staining in Normal tonsil tissue.

Product References

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