NFE2L2

Gene Symbol NFE2L2
Entrez Gene 4780
Alt Symbol NRF2
Species Human
Gene Type protein-coding
Description nuclear factor, erythroid 2-like 2
Other Description HEBP1|NF-E2-related factor 2|NFE2-related factor 2|nuclear factor (erythroid-derived 2)-like 2|nuclear factor erythroid 2-related factor 2|nuclear factor erythroid-derived 2-like 2|nuclear factor, erythroid derived 2, like 2
Swissprots Q59HH2 B4E338 E9PGJ7 B2RBU2 Q96F71 Q53RW6 Q16236
Accessions AAY14710 EAX11061 EAX11062 Q16236 AB208786 BAD92023 AF323119 AAK01657 AK297838 BAG60172 AK304555 BAG65350 AK310656 AK310841 AK311395 AK311688 AK314816 BAG37339 AK316148 BAH14519 AW471118 BC011558 AAH11558 BX649047 BX649071 CV575212 DA436387 DC314553 EU446639 ABZ92168 EU831991 ACE87432 EU832084 ACE86746 EU832824 ACE87835 GQ129228 ACT64362 HM446346 ADK56055 S74017 AAB32188 NM_001145412 NP_001138884 NM_001145413 NP_001138885 NM_006164 NP_006155
Function Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. {ECO:0000269|PubMed:11035812}.
Subcellular Location Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Tissue Specificity Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Top Pathways Protein processing in endoplasmic reticulum

NRF2 (D1Z9C) XP ® Rabbit mAb (PE Conjugate) - 14409 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D1Z9C
Flow cytometric analysis of MEF NRF2 wt cells, untreated (blue) and treated with MG-132 #2194 (green), using NRF2 (D1Z9C) XP ® Rabbit mAb (PE Conjugate).

NRF2 (D1Z9C) XP ® Rabbit mAb - 12721 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1Z9C
Western blot analysis of extracts from MEF wt and U-2 OS cells, untreated (-) or treated with MG-132 #2194 (10 μM, 10 hr; +), using NRF2 (D1Z9C) XP ® Rabbit mAb.

NRF2 (D1C9) Rabbit mAb - 8882 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D1C9

NRF2 Antibody - 4399 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Hep G2, 293 and COS cells using NRF2 Antibody.

Nrf2 (A-10) - sc-365949 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nrf2 (A-10): sc-365949. Western blot analysis of Nrf2 expression in Jurkat whole cell lysate (A) and THP-1 nuclear extract (B).

Nrf2 (C-20) - sc-722 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nrf2 (C-20): sc-722. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse kidney tissue showing nuclear localization.

Nrf2 (H-300) - sc-13032 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nrf2 (H-300): sc-13032. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Nrf2 (T-19) - sc-30915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nrf2 (T-19): sc-30915. Western blot analysis of Nrf2 expression in THP-1 nuclear extract.

Nrf2 (437C2a) - sc-81342 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Nrf2 (437C2a): sc-81342 Western Blot analysis of human recombinant Nrf2 fusion protein.

Anti-Nrf2 (phospho S40) antibody [EP1809Y] - ab76026 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1809Y
Overlay histogram showing Jurkat cells stained with ab76026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76026, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Nrf2 antibody - ab89443 from Abcam

Prices $387.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
IHC image of Nrf2 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89443, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Nrf2 antibody - ab97718 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of formalin-fixed, paraffin-embedded tissue labelled with ab97718 at 1/100 dilution.

Anti-Nrf2 antibody [437C2a] - ab54364 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 437C2a
Anti-Nrf2 antibody [437C2a] (ab54364) + immunised recombinant protein

Anti-Nrf2 antibody [EP1808Y] - ab62352 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1808Y
Anti-Nrf2 antibody [EP1808Y] (ab62352) at 1/2000 dilution + HepG2 cell lysate at 10 µgSecondarygoat anti rabbit HRP at 1/2000 dilution

Anti-Nrf2 antibody [EP1808Y] (Alexa Fluor® 488) - ab194984 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1808Y
Overlay histogram showing HeLa cells stained with ab194984 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab194984, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Nrf2 antibody [EP1808Y] (Alexa Fluor® 647) - ab194985 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1808Y
ab194985 staining Nrf2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab194985 at a working dilution 1/100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Nrf2 antibody [EP1808Y] (HRP) - ab194986 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1808Y
IHC image of Nrf2 staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab194986 at 1/500 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Nrf2 antibody - Aminoterminal end - ab75919 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab75919 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75919, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NFE2L2 / NRF2 Antibody (aa580-596) - LS-C312971 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
NFE2L2 / NRF2 antibody. IHC(P): Human Breast Cancer Tissue.

Anti-NFE2L2 / NRF2 Antibody - LS-C73767 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-NFE2L2 / NRF2 Antibody (aa569-588) - LS-C189130 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-NFE2L2 / NRF2 Antibody (N-Terminus, APC) - LS-C226029 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NFE2L2 / NRF2 Antibody (C-Terminus) - LS-C31314 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
NFE2L2 / NRF2 antibody LS-C31314 Western blot of A172 cell lysate.

Anti-NFE2L2 / NRF2 Antibody (Ser40) - LS-C100851 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of NFE2L2-S40 in T47D(lane 1) and 293(lane 2) cell line lysates (35 ug/lane). NFE2L2 (arrow) was detected using the purified antibody.

Anti-NFE2L2 / NRF2 Antibody (N-Terminus, AP) - LS-C226030 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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