ICC/IF image of ab54364 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54364, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-Nrf2 antibody [437C2a] (ab54364) + immunised recombinant protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2059_ab54364.gif)
Anti-Nrf2 antibody [437C2a] (ab54364) + immunised recombinant protein