Anti-Nrf2 antibody [EP1808Y]

• Supplier: Abcam
• Catalog: ab62352
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1808Y
• Applications: ChIP IHC-F ICC/IF ICC/IF WB IP IHC-P ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nrf2 aa 550 to the C-terminus (C terminal)
• Blocking Peptide: Nrf2 peptide
• Description: Rabbit Monoclonal
• Gene: NFE2L2
• Conjugate: Unconjugated

Product images

Anti-Nrf2 antibody [EP1808Y] (ab62352) at 1/2000 dilution + HepG2 cell lysate at 10 µgSecondarygoat anti rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of Nrf2 expression in paraffin embedded human lung adenocarcinoma using 1/100 ab62352

ICC/IF image of ab62352 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62352, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF image of ab62352 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62352, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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An J, Chen C, Wang X, Zhong Y, Zhang X, Yu Y, Yu Z. Toxicol In Vitro. 2014 Mar;28(2):319-26.

A novel acylaminoimidazole derivative, WN1316, alleviates disease progression via - A novel acylaminoimidazole derivative, WN1316, alleviates disease progression via

Tanaka K, Kanno T, Yanagisawa Y, Yasutake K, Inoue S, Hirayama N, Ikeda JE. PLoS One. 2014 Jan 31;9(1):e87728.

Modulation of nuclear factor E2-related factor-2 (Nrf2) activation by the stress - Modulation of nuclear factor E2-related factor-2 (Nrf2) activation by the stress

Stachel I, Geismann C, Aden K, Deisinger F, Rosenstiel P, Schreiber S, Sebens S, Arlt A, Schafer H. J Biol Chem. 2014 Jan 24;289(4):1917-29.

High-throughput and combinatorial gene expression on a chip for - High-throughput and combinatorial gene expression on a chip for

Kwon SJ, Lee DW, Shah DA, Ku B, Jeon SY, Solanki K, Ryan JD, Clark DS, Dordick JS, Lee MY. Nat Commun. 2014 May 6;5:3739.

Caffeic acid phenethylester increases stress resistance and enhances lifespan in - Caffeic acid phenethylester increases stress resistance and enhances lifespan in

Havermann S, Chovolou Y, Humpf HU, Watjen W. PLoS One. 2014 Jun 25;9(6):e100256.

Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2 - Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2

Zhao C, Gillette DD, Li X, Zhang Z, Wen H. J Biol Chem. 2014 Jun 13;289(24):17020-9.

In Barrett's esophagus patients and Barrett's cell lines, ursodeoxycholic acid - In Barrett's esophagus patients and Barrett's cell lines, ursodeoxycholic acid

Peng S, Huo X, Rezaei D, Zhang Q, Zhang X, Yu C, Asanuma K, Cheng E, Pham TH, Wang DH, Chen M, Souza RF, Spechler SJ. Am J Physiol Gastrointest Liver Physiol. 2014 Jul 15;307(2):G129-39. doi:

Coordinated regulation of protein synthesis and degradation by mTORC1. - Coordinated regulation of protein synthesis and degradation by mTORC1.

Zhang Y, Nicholatos J, Dreier JR, Ricoult SJ, Widenmaier SB, Hotamisligil GS, Kwiatkowski DJ, Manning BD. Nature. 2014 Sep 18;513(7518):440-3.

The effect of sulforaphane on histone deacetylase activity in keratinocytes: - The effect of sulforaphane on histone deacetylase activity in keratinocytes:

Dickinson SE, Rusche JJ, Bec SL, Horn DJ, Janda J, Rim SH, Smith CL, Bowden GT. Mol Carcinog. 2014 Oct 12.

Clinicopathologic significance of CXCR4 and Nrf2 in colorectal cancer. - Clinicopathologic significance of CXCR4 and Nrf2 in colorectal cancer.

Hu T, Yao Y, Yu S, Guo H, Han L, Wang W, Tian T, Hao Y, Liu Z, Nan K, Wang S. J Biomed Res. 2013 Jul;27(4):283-90.