ICC/IF image of ab75919 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75919, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Nrf2 antibody - Aminoterminal end (ab75919) at 1/60 dilution + Ramos cell line lysates at 35 µg
All lanes : Anti-Nrf2 antibody - Aminoterminal end (ab75919) at 1/50 dilutionLane 1 : 293 cell lysates, nontransfected Lane 2 : 293 cell lysates, transiently transfected with the Nrf2 gene Lysates/proteins at 2 µg per lane.