Anti-NSD3 antibody [EPR13813] - N-terminal

• Supplier: Abcam
• Catalog: ab180500
• Prices: $376.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13813
• Applications: WB FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NSD3 aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: WHSC1L1
• Conjugate: Unconjugated

Product images

Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/10000 dilution (purified)Lane 1 : Mouse spleen tissue lysateLane 2 : Rat spleen tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified)Lane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab180500 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of primary and secondary antibody were used as a negative control (blue line).

All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (unpurified)Lane 1 : Hela lysateLane 2 : A673 lysateLane 3 : MCF7 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Flow Cytometrical analysis of HeLa cells labeling NSD3 with unpurified ab180500 at 1/75 (green) compared to a negative control antibody (red).