WHSC1L1

Gene Symbol WHSC1L1
Entrez Gene 54904
Alt Symbol NSD3, pp14328
Species Human
Gene Type protein-coding
Description Wolf-Hirschhorn syndrome candidate 1-like 1
Other Description WHSC1-like 1 isoform 9 with methyltransferase activity to lysine|WHSC1-like protein 1|histone-lysine N-methyltransferase NSD3|nuclear SET domain-containing protein 3|protein whistle
Swissprots Q9BYU8 D3DSX1 Q9BZ95 Q9NXA6 Q1RMD3 Q9H9W9 B7ZL11 Q6ZSA5 Q9BYU9 Q3B796 Q9H2M8
Accessions EAW63319 EAW63320 EAW63321 EAW63322 Q9BZ95 AF255649 AAG44637 AF318339 AAL55846 AF332468 AAK00354 AF332469 AAK00355 AJ295990 CAC28350 AJ295991 CAC28351 AJ295992 CAC28352 AK000360 BAA91110 AK022560 BAB14099 AK023534 AK025702 AK092166 AK127594 AL832895 BC000030 BC007787 BC012059 AAH12059 BC062631 AAH62631 BC073858 BC101717 AAI01718 BC107734 AAI07735 BC113469 AAI13470 BC115006 AAI15007 BC115007 BC143510 AAI43511 JF432144 ADZ15361 XM_005273547 XP_005273604 XM_005273548 XP_005273605 XM_011544561 XP_011542863 XM_011544562 XP_011542864 XM_011544563 XP_011542865 NM_017778 NP_060248 NM_023034 NP_075447
Function Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. {ECO:0000269|PubMed:16682010}.
Subcellular Location Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
Tissue Specificity Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung. {ECO:0000269|PubMed:11374904}.
Top Pathways Lysine degradation

NSD3 (E-3) - sc-398186 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NSD3 (E-3): sc-398186. Western blot analysis of NSD3 expression in A-673 (A) and MCF7 (B) nuclear extracts and IMR-32 (C), LNCaP (D), HeLa (E) and HEK293 (F) whole cell lysates.

NSD3 (H-300) - sc-134817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NSD3 (H-300): sc-134817. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

NSD3 (N-20) - sc-50152 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSD3 (N-20): sc-50152. Western blot analysis of NSD3 expression in rat brain tissue extract.

NSD3 (H-8) - sc-271839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NSD3 (H-8): sc-271839. Western blot analysis of NSD3 expression in LNCaP whole cell lysate.

NSD3 (Q-20) - sc-50153 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSD3 (Q-20): sc-50153. Western blot analysis of NSD3 expression in rat brain tissue extract.

NSD3 (S-20) - sc-50154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSD3 (S-20): sc-50154. Western blot analysis of NSD3 expression in HeLa (A) and HEK293 (B) whole cell lysates.

NSD3 (C-15) - sc-50151 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NSD3 (C-15): sc-50151. Western blot analysis of NSD3 expression in HeLa (A) and IMR-32 (B) whole cell lysates.

NSD3 (2E9) - sc-130009 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
NSD3 (2E9): sc-130009. Western blot analysis of NSD3 expression in HeLa whole cell lysate.

Anti-NSD3 antibody - ab4514 from Abcam

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Breast carcinoma tissue was fixed in 10% formalin at 4°C overnight and paraffin embedded. Following blocking the tissue sections were incubated with ab4514 (1/50 dilution) at 37°C for 1 hour, and with the secondary antibody at 37°C for 30 minutes. The slides were counterstained with Hematoxylin. Washes were carried out using TBS. Heat mediated antigen retrieval was carried out using the Microwave method before commencing the staining protocol. ab4514 staining appears to be localized mainly to the cytoplasm. Although the putative cellular localization of NSD3 is the nucleus we have found no evidence in the current literature to confirm this and we believe ab4514 may be detecting a cytoplasmic form of the protein. We would be very grateful for any customer feedback about NSD3 and ab4514.

Anti-NSD3 antibody - ab137430 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling NSD3 with ab137430 at 1/200 dilution. Lower panel merged with DNA probe.

Anti-NSD3 antibody - ab176986 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of formalin-fixed, paraffin-embedded Human fetal colon tissue labeling NSD3 with ab176986 at 1/100 dilution showing cytoplasmic staining

Anti-NSD3 antibody [2E9] - ab66745 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E9
Overlay histogram showing HeLa cells stained with ab66745 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66745, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NSD3 antibody [EPR13813] - N-terminal - ab180500 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13813
Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-NSD3 antibody [N348/82] - ab167623 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone N348/82
All lanes : Anti-NSD3 antibody [N348/82] (ab167623) at 5 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NSD3 / WHSC1L1 Antibody (aa188-217) - LS-C160620 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of WHSC1L1 (arrow) using rabbit polyclonal WHSC1L1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the WHSC1L1 gene.

Anti-NSD3 / WHSC1L1 Antibody - LS-C334150 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of A549 cell using WHSC1L1 antibody. Blue: DAPI for nuclear staining.

Anti-NSD3 / WHSC1L1 Antibody (aa383-660, clone 2E9) - LS-C93773 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E9
Cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human NSD3 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-NSD3 / WHSC1L1 Antibody (aa383-660) - LS-C76860 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-NSD3 / WHSC1L1 Antibody (C-Terminus, AP) - LS-C273595 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSD3 / WHSC1L1 Antibody (C-Terminus, Biotin) - LS-C273597 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSD3 / WHSC1L1 Antibody (C-Terminus, APC) - LS-C273596 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSD3 / WHSC1L1 Antibody (C-Terminus, FITC) - LS-C273598 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSD3 / WHSC1L1 Antibody (C-Terminus, Azide-free, HRP) - LS-C273599 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSD3 / WHSC1L1 Antibody (C-Terminus, PE) - LS-C273600 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NSD3 / WHSC1L1 Antibody (C-Terminus) - LS-C326361 from LifeSpan Bioscience

Prices $435.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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