Anti-NSDHL antibody [EPR14489(2)]

• Supplier: Abcam
• Catalog: ab199730
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14489(2)
• Applications: WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NSDHL aa 100-200
• Description: Rabbit Monoclonal
• Gene: NSDHL
• Conjugate: Unconjugated

Product images

All lanes : Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilutionLane 1 : A431 (Human epidermoid carcinoma) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 4 : Human fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated) at 1/1000 dilution

All lanes : Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilutionLane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 3 : C6 (Rat glial tumor cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

NSDHL was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab199730 at 1/50 dilution. Lane 1: Human fetal brain whole cell lysate 10ug (Input).Lane 2: ab199730 IP in Human fetal brain whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199730 in Human fetal brain whole cell lysate.Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 5 seconds.

NSDHL was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab199730 at 1/50 dilution.Lane 1: HeLa whole cell lysate 10ug (Input).Lane 2: ab199730 IP in HeLa whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199730 in HeLa whole cell lysate.Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution (Panel A) or ab190353 at 1/1000 dilution (Panel B).Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds (Panel A and B). ab199730 was used to immunoprecipitate NSDHL. These precipitates were resolved with ab190353.