All lanes : Anti-NSE2 antibody [4A2] (ab119074) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY NSE2Lysates/proteins at 5 µg per lane.
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NSE2 using ab119074 at a dilution of 1/100.
HEK293T cells transfected with either NSE2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119074 at a dilution of 1/100, and then analyzed by flow cytometry.