Anti-Nuclear Factor Erythroid Derived 2 antibody (ab77321) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
All lanes : Anti-Nuclear Factor Erythroid Derived 2 antibody (ab77321) at 5 µg/mlLane 1 : Nuclear Factor Erythroid Derived 2 transfected 293T cell lysateLane 2 : Untransfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
ab77321 was used as a capture antibody and detection limit for recombinant tagged NFE2 is approximately 3ng/ml.