Anti-Nuclear Matrix Protein p84 antibody [5E10]
| Name | Anti-Nuclear Matrix Protein p84 antibody [5E10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab487 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 5E10 |
| Applications | WB IP ICC/IF ICC/IF ICC/IF IHC-P IHC-F FC |
| Species Reactivities | Mouse, Human |
| Antigen | Fusion protein containing amino acids 15-374 of human p84 expressed in E |
| Description | Mouse Monoclonal |
| Gene | THOC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilutionLane 1 : 0.1% DMSO treated Jurkat cell (whole cell lysate)Lane 2 : 0.5% DMSO treated Jurkat cell (whole cell lysate)Lane 3 : 1% DMSO treated Jurkat cell (whole cell lysate)Lane 4 : 0.1% SDS treated Jurkat cell (whole cell lysate)Lane 5 : 0.5% SDS treated Jurkat cell (whole cell lysate)Lane 6 : 1% SDS treated Jurkat cell (whole cell lysate)SecondaryHRP conjugated goat anti-mouse.developed using the ECL techniquePerformed under reducing conditions.Observed band size : 84 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2505_ab487_1.jpg)
All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilutionLane 1 : 0.1% DMSO treated Jurkat cell (whole cell lysate)Lane 2 : 0.5% DMSO treated Jurkat cell (whole cell lysate)Lane 3 : 1% DMSO treated Jurkat cell (whole cell lysate)Lane 4 : 0.1% SDS treated Jurkat cell (whole cell lysate)Lane 5 : 0.5% SDS treated Jurkat cell (whole cell lysate)Lane 6 : 1% SDS treated Jurkat cell (whole cell lysate)SecondaryHRP conjugated goat anti-mouse.developed using the ECL techniquePerformed under reducing conditions.Observed band size : 84 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview
ab487 staining Nuclear Matrix Protein p84 in Human stomach adenocarcinoma cell line (AGS) by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.025% Triton X-100, TBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 2µg/ml for 1 hour at 23°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 350 (blue) used undiluted.p84 shows nuclear localization.See Abreview
ICC/IF image of ab487 stained Hepp cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab487, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab487 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab487, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2508_Nuclear-Matrix-Protein-p84-Primary-antibodies-ab487-13.jpg)
Overlay histogram showing HeLa cells stained with ab487 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab487, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
.
Complex formation between S100B protein and the p90 ribosomal S6 kinase (RSK) in - Complex formation between S100B protein and the p90 ribosomal S6 kinase (RSK) in
Hartman KG, Vitolo MI, Pierce AD, Fox JM, Shapiro P, Martin SS, Wilder PT, Weber DJ. J Biol Chem. 2014 May 2;289(18):12886-95.
Dedifferentiation and aberrations of the endolysosomal compartment characterize - Dedifferentiation and aberrations of the endolysosomal compartment characterize
Raggi C, Luciani A, Nevo N, Antignac C, Terryn S, Devuyst O. Hum Mol Genet. 2014 May 1;23(9):2266-78.
FK506 reduces calpain-regulated calcineurin activity in both the cytoplasm and - FK506 reduces calpain-regulated calcineurin activity in both the cytoplasm and
Lee SH, Choi J, Kim H, Lee DH, Roh GS, Kim HJ, Kang SS, Choi WS, Cho GJ. Anat Cell Biol. 2014 Jun;47(2):91-100.
SH3BP2 gain-of-function mutation exacerbates inflammation and bone loss in a - SH3BP2 gain-of-function mutation exacerbates inflammation and bone loss in a
Mukai T, Gallant R, Ishida S, Yoshitaka T, Kittaka M, Nishida K, Fox DA, Morita Y, Ueki Y. PLoS One. 2014 Aug 21;9(8):e105518.
miR-106b downregulates adenomatous polyposis coli and promotes cell proliferation - miR-106b downregulates adenomatous polyposis coli and promotes cell proliferation
Shen G, Jia H, Tai Q, Li Y, Chen D. Carcinogenesis. 2013 Jan;34(1):211-9.
Pasteurella multocida toxin prevents osteoblast differentiation by - Pasteurella multocida toxin prevents osteoblast differentiation by
Siegert P, Schmidt G, Papatheodorou P, Wieland T, Aktories K, Orth JH. PLoS Pathog. 2013;9(5):e1003385.
Oxidative stress and Nrf2 signaling in McArdle disease. - Oxidative stress and Nrf2 signaling in McArdle disease.
Kitaoka Y, Ogborn DI, Nilsson MI, Mocellin NJ, MacNeil LG, Tarnopolsky MA. Mol Genet Metab. 2013 Nov;110(3):297-302.
Reactive oxygen species regulate context-dependent inhibition of NFAT5 target - Reactive oxygen species regulate context-dependent inhibition of NFAT5 target
Kim NH, Hong BK, Choi SY, Moo Kwon H, Cho CS, Yi EC, Kim WU. Exp Mol Med. 2013 Jul 19;45:e32.
DISC1 variants 37W and 607F disrupt its nuclear targeting and regulatory role in - DISC1 variants 37W and 607F disrupt its nuclear targeting and regulatory role in
Malavasi EL, Ogawa F, Porteous DJ, Millar JK. Hum Mol Genet. 2012 Jun 15;21(12):2779-92.