THOC1

Gene Symbol THOC1
Entrez Gene 9984
Alt Symbol HPR1, P84, P84N5
Species Human
Gene Type protein-coding
Description THO complex 1
Other Description THO complex subunit 1|hTREX84|nuclear matrix protein p84|tho1
Swissprots Q15219 Q64I72 Q96FV9 Q64I73 B2RBP6
Accessions EAX01731 EAX01732 EAX01733 EAX01734 Q96FV9 AK055354 AK123530 AK225067 AK225709 AK314755 BAG37293 AY573302 AAT81408 AY573303 AAT81409 BC010381 AAH10381 DA902294 DQ892768 ABM83694 EU176747 ABW03548 L36529 AAA53571 XM_011525772 XP_011524074 XM_011525773 XP_011524075 XM_011525774 XP_011524076 NM_005131 NP_005122
Function Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.
Subcellular Location Isoform 1: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
Tissue Specificity Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. {ECO:0000269|PubMed:15358532, ECO:0000269|PubMed:15833825}.
Top Pathways Spliceosome, RNA transport

THOC1 (29) - sc-136426 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
THOC1 (29): sc-136426. Western blot analysis of THOC1 expression in Jurkat whole cell lysate.

THOC1 (E-10) - sc-514123 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
THOC1 (E-10): sc-514123. Western blot analysis of THOC1 expression in Jurkat (A), MDA-MB-231 (B), MDA-MB-468 (C), ZR-75-1 (D) and MCF7 (E) whole cell lysates.

THOC1 (S-20) - sc-69035 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Nuclear Matrix Protein p84 antibody - ab102684 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Nuclear Matrix Protein p84 antibody (ab102684) at 1/1000 dilution + A549 whole cell lysate at 30 µg

Anti-Nuclear Matrix Protein p84 antibody - ab124180 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Nuclear Matrix Protein p84 antibody (ab124180) at 1/100 dilution + Y79 cell line lysate at 35 µg

Anti-Nuclear Matrix Protein p84 antibody - ab192794 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling Nuclear Matrix Protein p84 with ab192794 at 7.5 µg/ml.

Anti-Nuclear Matrix Protein p84 antibody [3121C2a] - ab54370 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3121C2a
Anti-Nuclear Matrix Protein p84 antibody [3121C2a] (ab54370) + immunising recombinant fragment

Anti-Nuclear Matrix Protein p84 antibody [5E10] - ab487 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5E10
All lanes : Anti-Nuclear Matrix Protein p84 antibody [5E10] (ab487) at 1/1000 dilutionLane 1 : 0.1% DMSO treated Jurkat cell (whole cell lysate)Lane 2 : 0.5% DMSO treated Jurkat cell (whole cell lysate)Lane 3 : 1% DMSO treated Jurkat cell (whole cell lysate)Lane 4 : 0.1% SDS treated Jurkat cell (whole cell lysate)Lane 5 : 0.5% SDS treated Jurkat cell (whole cell lysate)Lane 6 : 1% SDS treated Jurkat cell (whole cell lysate)SecondaryHRP conjugated goat anti-mouse.developed using the ECL techniquePerformed under reducing conditions.Observed band size : 84 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-Nuclear Matrix Protein p84 antibody [5E10] - ab94812 from Abcam

Prices $372.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 5E10

Anti-Nuclear Matrix Protein p84 antibody [EPR5661] - ab125019 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5661
All lanes : Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019) at 1/1000 dilutionLane 1 : Hela cell lysateLane 2 : U-87 MG cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] - ab131268 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5662(2)
All lanes : Anti-Nuclear Matrix Protein p84 antibody [EPR5662(2)] (ab131268) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : U-87 MG cell lysateLane 3 : 293T cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

hHpr1-p84-Thoc1 Antibody (5E10) - NB100-174 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5E10
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

hHpr1-p84-Thoc1 Antibody - NBP1-89669 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

hHpr1-p84-Thoc1 Antibody - NBP1-47304 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- THOC1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate

hHpr1-p84-Thoc1 Antibody - NBP2-16822 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.

Nuclear Matrix Protein p84 Antibody - bs-2448R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit

Anti-THOC1 antibody produced in rabbit - AV40578 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-THOC1: <B>Cat. No. AV40578</B>: Immunohistochemistry of THOC1 in Murine Small Intestine tissue with THOC1 antibody at 1.0 μg/mL.

Monoclonal Anti-THOC1 antibody produced in mouse - SAB2702154 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Mouse
<B>Immunoblotting</B><BR/>Monoclonal Anti-THOC1: <B>Cat. No. SAB2702154</B>: Western blot analysis of THOC1 in extracts from 30 μg of whole cell: <BR/><B>Lane</B><BR/>A) 293T; <BR/><B>Lane</B><BR/>B) A431; <BR/><B>Lane</B><BR/>C) HeLa; <BR/><B>Lane</B><BR/>D) HepG2; <BR/><B>Lane</B><BR/>E) A375 7.5% SDS-PAGE at a 1:500 dilution.

Anti-THOC1 antibody produced in rabbit - HPA019096 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-THOC1: <B>Cat. No. HPA019096</B>: Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Anti-THOC1 antibody produced in rabbit - HPA019687 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-THOC1: <B>Cat. No. HPA019687</B>: Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Anti-THOC1 antibody produced in rabbit - SAB2701984 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-THOC1: <B>Cat. No. SAB2701984</B>: Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Nuclear Matrix Protein p84 antibody at a 1:500 dilution.

THOC1 Antibody - N-terminal region (Ascites) (OAAB06870) - OAAB06870 from Aviva Systems Biology

Prices $320.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 301CT40.5.1

THOC1 Antibody - N-terminal region (OAAB06871) - OAAB06871 from Aviva Systems Biology

Prices $320.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 301CT40.5.1

THOC1 Antibody - C-terminal region (ARP40577_P050) - ARP40577_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

THOC1 antibody - C-terminal region (ARP40578_P050) - ARP40578_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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