Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab3482
• Prices: $395.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP WB ChIP IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Rat, Xenopus, Xenopus
• Antigen: Synthetic peptide corresponding to Mouse Nuclear Receptor Corepressor NCoR aa 2427-2443
• Description: Rabbit Polyclonal
• Gene: NCOR1
• Conjugate: Unconjugated

Product images

ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Mouse breast tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

Product References

Stage-specific functions of leukemia/lymphoma-related factor (LRF) in the - Stage-specific functions of leukemia/lymphoma-related factor (LRF) in the

Tsuji-Takechi K, Negishi-Koga T, Sumiya E, Kukita A, Kato S, Maeda T, Pandolfi PP, Moriyama K, Takayanagi H. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2561-6. doi:

4-Hydroxy-PCB106 acts as a direct thyroid hormone receptor agonist in rat GH3 - 4-Hydroxy-PCB106 acts as a direct thyroid hormone receptor agonist in rat GH3

You SH, Gauger KJ, Bansal R, Zoeller RT. Mol Cell Endocrinol. 2006 Sep 26;257-258:26-34. Epub 2006 Aug 23.