Anti-Nucleolin antibody

• Supplier: Abcam
• Catalog: ab70493
• Prices: $398.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Human, Rabbit, Horse, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Monkey, Ape, Monkey, Orangutan, Elephant
• Antigen: Synthetic peptide corresponding to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin
• Description: Rabbit Polyclonal
• Gene: NCL
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Nucleolin with ab70493 at 1/1000 (0.2µg/ml). Detection: DAB.

All lanes : Anti-Nucleolin antibody (ab70493) at 0.02 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from HeLa cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

Detection of Human Nucleolin by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70493 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of nucleolin was performed using ab70493 at 1 µg/ml.

ICC/IF image of ab70493 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70493, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab70493 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70493, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

The maternal nucleolus plays a key role in centromere satellite maintenance - The maternal nucleolus plays a key role in centromere satellite maintenance

Fulka H, Langerova A. Development. 2014 Apr;141(8):1694-704.

ANKRD1 acts as a transcriptional repressor of MMP13 via the AP-1 site. - ANKRD1 acts as a transcriptional repressor of MMP13 via the AP-1 site.

Almodovar-Garcia K, Kwon M, Samaras SE, Davidson JM. Mol Cell Biol. 2014 Apr;34(8):1500-11.

Nucleolin mediates nucleosome disruption critical for DNA double-strand break - Nucleolin mediates nucleosome disruption critical for DNA double-strand break

Goldstein M, Derheimer FA, Tait-Mulder J, Kastan MB. Proc Natl Acad Sci U S A. 2013 Oct 15;110(42):16874-9. doi: