NCL

Gene Symbol NCL
Entrez Gene 4691
Alt Symbol C23
Species Human
Gene Type protein-coding
Description nucleolin
Other Description -
Swissprots Q9UCF0 Q53SK1 Q9UDG1 Q8NB06 P19338
Accessions AAA59954 AAY24247 CCQ43318 EAW70960 EAW70961 EAW70962 EAW70963 P19338 AK000221 AK000250 AK001314 BAG50892 AK091742 BAC03738 AK095897 BAG53161 AK122805 AK127608 BAC87055 AK128584 AY320411 BC002343 AAH02343 BC006494 AAH06494 BC006516 AAH06516 BM761058 BQ001029 NM_005381 NP_005372
Function Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. {ECO:0000269|PubMed:10393184}.
Subcellular Location Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Top Pathways Pathogenic Escherichia coli infection

Nucleolin (D4C7O) Rabbit mAb - 14574 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4C7O
Western blot analysis of extracts from various cell lines using Nucleolin (D4C7O) Rabbit mAb.

Nucleolin Antibody - 12247 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

C23 (D-6) - sc-17826 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C23 (D-6): sc-17826. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing nuclear localization.

C23 (F-18) - sc-9893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
C23 (F-18): sc-9893. Western blot analysis of C23 expression in CCRF-CEM (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.

C23 (H-250) - sc-13057 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
C23 (H-250): sc-13057. Western blot analysis of C23 expression in KNRK nuclear extract.

C23 (K-18) - sc-34400 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
C23 (K-18): sc-34400. Western blot analysis of C23 expression in NIH/3T3 (A) and SH-SY5Y (B) whole cell lysates.

C23 (MS-3) - sc-8031 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C23 (MS-3): sc-8031. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing nuclear localization.

C23 (H-6) - sc-55486 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
C23 (H-6): sc-55486. Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar localization.

C23 (K-14) - sc-34399 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
C23 (K-14): sc-34399. Western blot analysis of C23 expression in Jurkat (A), K-562 (B) and CCRF-CEM (C) whole cell lysates.

Anti-Nucleolin (acetyl K88) antibody - ab196345 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Nucleolin (phospho T76) antibody - ab196348 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Nucleolin (phospho T76) antibody [ EPR8080-2] - ab168363 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8080-2
ab168363 showing +ve staining in Human ovarian carcinoma.

Anti-Nucleolin (phospho T84) antibody - ab196338 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Nucleolin (phospho T84) antibody [EPR8080] - ab155977 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8080
All lanes : Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) at 1/1000 dilutionLane 1 : 293T cell lysate, untreatedLane 2 : 293T cell lysate treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.

Anti-Nucleolin antibody - ab22758 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Nucleolin was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Nucleolin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22758.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 100kDa: Nucleolin.

Anti-Nucleolin antibody - ab50279 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Indirect Immunofluorescence Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.

Anti-Nucleolin antibody - ab70493 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Nucleolin with ab70493 at 1/1000 (0.2µg/ml). Detection: DAB.

Anti-Nucleolin antibody - ab170518 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HeLa cells, labeling Nucleolin using ab170518 at a 1/25 dilution. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).

Anti-Nucleolin antibody [130CT38.1.5] - ab86727 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 130CT38.1.5
IHC image of ab86727 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86727, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Nucleolin antibody [364-5] - ab136649 from Abcam

Prices $397.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 364-5
ab0.3316 staining Nucleolin in human breast cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 1 hour. An undiluted Alexa Fluor® 546-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview

Anti-Nucleolin antibody [364-5] (Alexa Fluor® 488) - ab154028 from Abcam

Prices $431.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 364-5
Immunocytochemistry with HeLa cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI (1/10,000) in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HeLa cells 40x. B) HeLa cells 100x.

Anti-Nucleolin antibody [364-5] (Alexa Fluor® 647) - ab198580 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 364-5
ab198580 staining Nucleolin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198580 at 1/100 dilution (shown in red) and ab6046 (Rabbit polyclonal to beta Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed, at 1µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Nucleolin antibody [364-5] (HRP) - ab198492 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 364-5
IHC image of Nucleolin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198492 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Nucleolin antibody [4E2] - ChIP Grade - ab13541 from Abcam

Prices $403.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4E2
ab 13541 at a 1/2000 dilution staining U2OS cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Alexa-Fluor ® 594 chicken anti-mouse antibody. This image is courtesy of an Abreview submitted by Raffaela Santoro on 16 March 2006.See Abreview

Anti-Nucleolin antibody [4i51] - ab14966 from Abcam

Prices $384.00
Sizes 25 µl
Host Mouse
Clonality Monoclonal
Clone 4i51
Anti-Nucleolin antibody [4i51] (ab14966) at 1/5000 dilution + Hela nuclear lysatedeveloped using the ECL technique
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