Immunocytochemistry with HeLa cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI (1/10,000) in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HeLa cells 40x. B) HeLa cells 100x.
Immunocytochemistry with HDFn cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HDFn 40x. B) HDFn 100x.
Flow Cytometry for Anti-Nucleolin-488 antibody (ab154028) stained HeLa cells. Flow cytometry results of unstained cells (black) and 1.0 µg/mL Nucleolin-488 (red) stained cells.