Anti-Nucleolin antibody [4E2] - ChIP Grade
| Name | Anti-Nucleolin antibody [4E2] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab13541 |
| Prices | $403.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4E2 |
| Applications | ICC/IF ICC/IF IHC-P WB ICC/IF FC ChIP |
| Species Reactivities | Human |
| Antigen | Human nucleolin protein from Raji cell extract |
| Description | Mouse Monoclonal |
| Gene | NCL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab 13541 at a 1/2000 dilution staining U2OS cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Alexa-Fluor ® 594 chicken anti-mouse antibody. This image is courtesy of an Abreview submitted by Raffaela Santoro on 16 March 2006.See Abreview
ab13541 at 1µg/ml staining Nucleolin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
![All lanes : Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) at 1 µg/mlLane 1 : Cell lysates prepared from human HL60 cellsLane 2 : Cell lysates prepared from human ZR-75-1 cells](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2606_Nucleolin-Primary-antibodies-ab13541-2.jpg)
All lanes : Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) at 1 µg/mlLane 1 : Cell lysates prepared from human HL60 cellsLane 2 : Cell lysates prepared from human ZR-75-1 cells
![Overlay histogram showing HeLa cells stained with ab13541 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13541, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2607_Nucleolin-Primary-antibodies-ab13541-3.jpg)
Overlay histogram showing HeLa cells stained with ab13541 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13541, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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