Anti-Nuf2 antibody [EPR13018(B)]

• Supplier: Abcam
• Catalog: ab176556
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13018(B)
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Nuf2 aa 1-100 (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: NUF2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Nuf2 antibody [EPR13018(B)] (ab176556) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Human fetal liver lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.developed using the ECL technique

ab176556 staining Nuf2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.See Abreview

Flow cytometry analysis of permeabilized 293T cells labeling Nuf2 (red), using ab176556 at a 1/10 dilution, or negative control rabbit IgG (green)