NUF2

Gene Symbol NUF2
Entrez Gene 83540
Alt Symbol CDCA1, CT106, NUF2R
Species Human
Gene Type protein-coding
Description NUF2, NDC80 kinetochore complex component
Other Description NUF2, NDC80 kinetochore complex component, homolog|cancer/testis antigen 106|cell division cycle associated 1|cell division cycle-associated protein 1|hNuf2|hNuf2R|hsNuf2|kinetochore protein Nuf2
Swissprots Q96HJ4 Q9BZD4 Q96Q78 Q8WU69
Accessions EAW90735 EAW90736 EAW90737 Q9BZD4 AB050577 BAB59141 AB050578 BAB59142 AF326731 AAK01426 AK093348 BAC04140 BC008489 AAH08489 BC021171 AAH21171 BI545465 DQ893170 ABM84096 DQ896469 ABM87468 XM_011510036 XP_011508338 NM_031423 NP_113611 NM_145697 NP_663735
Function Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. {ECO:0000269|PubMed:12438418, ECO:0000269|PubMed:14654001, ECO:0000269|PubMed:15062103, ECO:0000269|PubMed:15235793, ECO:0000269|PubMed:15239953, ECO:0000269|PubMed:15548592, ECO:0000269|PubMed:17535814}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization.

CdcA1 (E-6) - sc-271251 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CdcA1 (E-6): sc-271251. Western blot analysis of CdcA1 expression in HeLa (A) and T24 (B) whole cell lysates.

CdcA1 (H-300) - sc-98369 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

CdcA1 (N-13) - sc-103429 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CdcA1 (28-37) - sc-53635 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-Nuf2 antibody - ab122962 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Nuf2 antibody (ab122962) at 1/5000 dilution + HeLa cell lysate

Anti-Nuf2 antibody - ab96147 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Nuf2 antibody (ab96147) at 1/3000 dilution + A431 whole cell lysate at 30 µg

Anti-Nuf2 antibody - ab172706 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-Nuf2 antibody (ab172706) at 1/500 dilutionLane 1 : Nuf2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat anti-Mouse HRP at 1/2500 dilution

Anti-Nuf2 antibody [EPR13018(B)] - ab176556 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13018(B)
All lanes : Anti-Nuf2 antibody [EPR13018(B)] (ab176556) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Human fetal liver lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.developed using the ECL technique

Anti-Nuf2 antibody [EPR13019] - ab180945 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13019
All lanes : Anti-Nuf2 antibody [EPR13019] (ab180945) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : 293 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Nuf2 antibody - C-terminal - ab187682 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nuf2 antibody - C-terminal (ab187682) at 0.4 µg/mlLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : TCMK-1, whole cell lysateLane 5 : NIH3T3 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique

Anti-Nuf2 antibody - N-terminal - ab186561 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Nuf2 antibody - N-terminal (ab186561) at 1 µg/ml + RPMI-8226 whole cell lysate at 10 µg

Anti-CDCA1 / NUF2 Antibody (aa375-425) - LS-C290956 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CDCA1 / NUF2 Antibody (aa414-464) - LS-C290959 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse NUF2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-CDCA1 / NUF2 Antibody (clone 4H9) - LS-C179846 from LifeSpan Bioscience

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H9

Anti-CDCA1 / NUF2 Antibody (aa13-62) - LS-C206144 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CDCA1 / NUF2 antibody Western Blot of RPMI-8226. Antibody dilution: 1 ug/ml.

Anti-CDCA1 / NUF2 Antibody - LS-C186172 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS using CDCA1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Nuf2 Antibody - NBP1-31632 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Nuf2 Antibody - NBP2-32211 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Nuf2 Antibody - H00083540-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: Nuf2 Antibody [H00083540-B01] - analysis of CDCA1 expression in transfected 293T cell line by CDCA1 MaxPab polyclonal antibody (B01). Lane 1: CDCA1 transfected lysate (54.30 KDa). Lane 2: Non-transfected lysate.

Nuf2 Antibody - H00083540-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: Nuf2 Antibody [H00083540-A01]

Nuf2 Antibody - NBP2-32210 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: Nuf2 Antibody [NBP2-32210] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32210 used for IP at 6 ug per reaction. For blotting immunoprecipitated NUF2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

CDCA1 Polyclonal Antibody - bs-7714R from Bioss

Prices $279.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti CDCA1/Nuf2 Polyclonal Antibody, Unconjugated (bs-7714R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Monoclonal Anti-Nuf2 antibody produced in mouse - N5287 from Sigma-Aldrich

Prices $466.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 28-37

Anti-NUF2 (261-275) antibody produced in rabbit - SAB1105739 from Sigma-Aldrich

Prices $279.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human NUF2 was separated on SDS-PAGE, blotted with Anti-NUF2 (261-275) (<B>Cat. No. SAB1105739</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250

Anti-NUF2 antibody produced in rabbit - SAB2700437 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-NUF2: <B>Cat. No. SAB2700437</B>: Immunofluorescence analysis of paraformaldehyde-fixed U2OS, using CDCA1 antibody <B>(Geen)</B>at 1:500 dilution. α-Tubulin filaments were labeled with α-Tubulin antibody (Red) at 1:2000.
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