Anti-NUMB antibody
| Name | Anti-NUMB antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab14140 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF IHC-F IHC WB IP |
| Species Reactivities | Mouse, Rat, Human, Zebrafish, Chicken, Monkey, Primate |
| Antigen | Synthetic peptide derived from residues 600 to the C-terminus of Human NUMB |
| Blocking Peptide | Human NUMB peptide |
| Description | Rabbit Polyclonal |
| Gene | NUMB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab14140 staining NUMB in Zebrafish embryo nervous system by Immunohistochemistry (Whole mount). Samples were incubated with primary antibody (1/500 in BSA blocking buffer) for 48 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.See Abreview
All lanes : Anti-NUMB antibody (ab14140) at 1 µg/mlLane 1 : Brain (Rat) Tissue LysateLane 2 : Brain (Mouse) Tissue LysateLane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-NUMB antibody (ab14140) at 0.5 µg/ml + Adult Rat whole brain lysate at 40 µgSecondaryGoat anti-rabbit IgG HRP at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-NUMB antibody (ab14140) at 0.5 µg/ml + Mouse whole brain lysateSecondaryGoat anti-rabbit IgG HRP at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![NUMB was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to NUMB and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab14140.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 75kDa: NUMB.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2925_NUMB-Primary-antibodies-ab14140-21.jpg)
NUMB was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to NUMB and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab14140.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 75kDa: NUMB.
ab14140 detecting NUMB protein in dissociated neural precursor cells cultured from embryonic day 13 mouse neocortex in the presence of bFGF, EGF and LIF. NUMB immunoreactivity (green) was found in the cytosol of neural precursor cells. This localisation is in general agreement with published studies (e.g. Chen et al., EJN, 2005), where similar labelling is observed in the cytosol of cells of the grey matter of adult mouse spinal cord. Immunocytochemistry: All steps were performed in PBS. Cells were fixed in 4% PFA for 15min, permeabilised with 0.1% TX100 for 10min and blocked with 5% BSA, 0.1% TX100 for 45min. ab14140 was incubated at 8µg/ml for 12h at 4°C in 5% BSA, 0.1% TX100. Cultures were washed (3x) of primary antibody solution. Goat anti-rabbit AlexaFluor 488 was used as secondary antibody (1/400) in 5% BSA, 0.1% TX100 for 1h at RT. To-pro-3 was used as a nuclear counterstain (blue). Treated cultures were mounted on glass coverslips with Mowiol.
ab14140 at 1/100 staining rat brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 24 hours. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary. NUMB staining is shown in green.See Abreview
Image courtesy of Human Protein Atlasab14140 staining NUMB in Human adrenal gland. The paraffin embedded human tissue was incubated with ab14140 (1/250 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab14140 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.Further results for this antibody can be found at www.proteinatlas.org
Performed under reducing conditions.
Performed under reducing conditions.
Performed under reducing conditions.
Product References
Numb family proteins are essential for cardiac morphogenesis and progenitor - Numb family proteins are essential for cardiac morphogenesis and progenitor
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Expression and function of NUMB in odontogenesis. - Expression and function of NUMB in odontogenesis.
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Angiotensin II inhibits satellite cell proliferation and prevents skeletal muscle - Angiotensin II inhibits satellite cell proliferation and prevents skeletal muscle
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Interleukin-10 regulates progenitor differentiation and modulates neurogenesis in - Interleukin-10 regulates progenitor differentiation and modulates neurogenesis in
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Numb expression and asymmetric versus symmetric cell division in distal embryonic - Numb expression and asymmetric versus symmetric cell division in distal embryonic
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The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch - The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch
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Functional dicer is necessary for appropriate specification of radial glia during - Functional dicer is necessary for appropriate specification of radial glia during
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Epicardial spindle orientation controls cell entry into the myocardium. - Epicardial spindle orientation controls cell entry into the myocardium.
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