All lanes : Anti-NUMB antibody [1A7] (ab123891) at 1/2000 dilutionLane 1 : lysate from HEK293T cells transfected with the pCMV6-ENTRY control cDNALane 2 : lysate from HEK293T cells transfected with the pCMV6-ENTRY NUMB cDNALysates/proteins at 5 µg per lane.
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB using 1/100 ab123891.
HEK293T cells transfected with either NUMB overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab123891 at 1/100 dilution, and then analyzed by flow cytometry.