Anti-Nup153 antibody [QE5]
| Name | Anti-Nup153 antibody [QE5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24700 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | QE5 |
| Applications | FC WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Full length protein: rat liver nuclear envelope proteins |
| Description | Mouse Monoclonal |
| Gene | NUP153 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab24700 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24700, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Methanol fixed HeLa stained with ab24700. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with Giantin (yellow).
Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 4% formaldehyde and permeabilized in 0.2% Triton x100 for 10 minutes at room temperature, then washed 3 times in PBS. The fixation and permeabilization was carried out in a single step. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).A: HeLa cells, ab24700 used at 4µg/mlB: 3T3 cells, ab24700 used at 2µg/mlC: 3T3 cells, ab24700 used at 4µg/mlD: 3T3 cells, ab24700 used at 6.6µg/ml
Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 100% methanol for 20 minutes at -20°C, then washed once in PBS. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).A: HeLa cells, ab24700 used at 4µg/mlB: 3T3 cells, ab24700 used at 2µg/mlC: 3T3 cells, ab24700 used at 4µg/mlD: 3T3 cells, ab24700 used at 6.6µg/ml
![Overlay histogram showing HeLa cells stained with ab24700 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24700, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2988_Nup153-Primary-antibodies-ab24700-3.jpg)
Overlay histogram showing HeLa cells stained with ab24700 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24700, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Anti-Nup153 antibody [QE5] (ab24700) at 1/1000 dilution + Human U2OS nuclear lysate at 40 µgSecondaryHRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/2989_ab24700-222317-ab24700wb.jpg)
Anti-Nup153 antibody [QE5] (ab24700) at 1/1000 dilution + Human U2OS nuclear lysate at 40 µgSecondaryHRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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