Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling NUP50 with ab85915 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-NUP50 antibody (ab85915) at 0.04 µg/mlLane 1 : HeLa whole cell lysate. at 50 µgLane 2 : HeLa whole cell lysate. at 15 µgLane 3 : HeLa whole cell lysate. at 5 µg
Detection of NUP50 by Western Blot of Immunprecipitate. ab85915 at 1µg/ml staining NUP50 in HeLa whole cell lysate immunoprecipitated using ab85915 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.