All lanes : Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution.
ab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview