All lanes : Anti-Nurr1 antibody (ab93332) at 1/1000 dilutionLane 1 : MW markerLane 2 : Human hippocampus tissue lysate at 20 µgLane 3 : Human hippocampus tissue lysate at 20 µg with immunizing peptideLane 4 : Human hippocampus tissue lysate at 20 µgSecondaryAP-conjugated secondary antibody at 1/5000 dilution
ab93332 at 1/100 staining Nurr1 in 20 µm thick sections of formalin-fixed adult Rat hippocampus tissue.
ab93332 staining Nurr1 in human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and an enzymatic antigen retrieval step was performed using Proteinase K. Samples were then incubated with ab93332 at a 1/250 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/ rabbit polyclonal.See Abreview