All lanes : Anti-NXN [EPR15177] antibody (ab191429) at 1/10000 dilutionLane 1 : 293 lysateLane 2 : HepG2 lysateLane 3 : HeLa lysateLane 4 : SH-SY5Y lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling NXN with ab191429 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-NXN) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution; 2. Primary ab concentration (anti-NXN) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NXN with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody.
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling NXN with ab191429 at 1/100 with prediluted HRP Polymer for Rabbit IgG as secondary antibody. Negative control: Using PBS instead of primary antibody.
All lanes : Anti-NXN [EPR15177] antibody (ab191429) at 1/10000 dilutionLane 1 : Mouse testis lysateLane 2 : Rat testis lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution