Anti-OAS1 antibody
| Name | Anti-OAS1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab82666 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide from within a region of C terminal amino acids 323-372 (EAWLNYPCFK NWDGSPVSSW ILLAESNSAD DETDDPRRYQ KYGYIGTHEY) of Human OAS1 (NP_058132) Run BLAST with Run BLAST with |
| Blocking Peptide | OAS1 peptide |
| Description | Rabbit Polyclonal |
| Gene | OAS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab82666 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-OAS1 antibody (ab82666) at 1 µg/ml (5% skim milk/ PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 12%SecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
Immunohistochemistry of Tonsil tissue at an antibody concentration of 5µg/ml using anti-OAS1 antibody (ab82666)