Anti-Oct4 antibody
| Name | Anti-Oct4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18976 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB IP ICC/IF ICC/IF FC IHC-P |
| Species Reactivities | Mouse, Rat, Human, Pig, Monkey, Chimpanzee |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) |
| Blocking Peptide | Oct4 peptide |
| Description | Rabbit Polyclonal |
| Gene | POU5F1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab18976 at 1/100 dilution staining Oct-4 in mouse brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% BSA for 1 hour at 25°C and then incubated with ab18976 for 12 hours at 4°C. A Cy3 conjugated donkey polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody.See Abreview
Immunohistochemical analysis of paraffin embedded Human breast tissues (5 µm thick) labeling Oct4 with ab18976 at 1:100 to 1:200 dilution. Heat induced epitope retrieval was performed at 100°C for 30 min. Slides were counterstained with hematoxylin.Minimal and mostly cytoplasmic expression was seen in the normal resting breast (left panel). During normal lactation (right panel), OCT4 was upregulated and expressed in both the nucleus and cytoplasm.
Immunofluorescent analysis of paraffin embedded Human breast tissues (5 µm thick) labeling Oct4 with ab18976 at 1:100 dilution.Tissues were rehydrated in deionized water and incubated in PBS prior to per meabilization in 0.1% PBS-TritonX 100 and overnight incubation with ab18976 in a humid chamber.Washing in PBS and secondary antibody (Alexa fluor) incubation for 2h was followed by a final wash and mounting.Blue: DAPI stain for nuclei.Minimal and mostly cytoplasmic expression was seen in the normal resting breast (left panel). During normal lactation (right panel), OCT4 was upregulated and expressed in both the nucleus and cytoplasm.
Anti-Oct4 antibody (ab18976) at 1/500 dilution + mouse embryonic stem cell whole cell lysate at 100 µg/mlSecondaryHRP conjugated goat anti-rabbitdeveloped using the ECL techniquePerformed under reducing conditions.
Anti-Oct4 antibody (ab18976) at 0.5 µg/ml + Lysates prepared from rat kidney tissue.
Product References
Expression and differentiation between OCT4A and its Pseudogenes in human ESCs - Expression and differentiation between OCT4A and its Pseudogenes in human ESCs
Jez M, Ambady S, Kashpur O, Grella A, Malcuit C, Vilner L, Rozman P, Dominko T. PLoS One. 2014 Feb 24;9(2):e89546.
Immunohistochemical expression of four different stem cell markers in prostate - Immunohistochemical expression of four different stem cell markers in prostate
Miyazawa K, Tanaka T, Nakai D, Morita N, Suzuki K. Oncol Lett. 2014 Sep;8(3):985-992. Epub 2014 Jun 23.
Exogenous expression of OCT4 facilitates oocyte-mediated reprogramming in cloned - Exogenous expression of OCT4 facilitates oocyte-mediated reprogramming in cloned
Ji Q, Cong P, Zhao H, Song Z, Zhao G, Gao J, Nie Y, Chen Y. Mol Reprod Dev. 2014 Sep;81(9):820-32.
Expression of the Pluripotency Transcription Factor OCT4 in the Normal and - Expression of the Pluripotency Transcription Factor OCT4 in the Normal and
Hassiotou F, Hepworth AR, Beltran AS, Mathews MM, Stuebe AM, Hartmann PE, Filgueira L, Blancafort P. Front Oncol. 2013 Apr 11;3:79.
Medullospheres from DAOY, UW228 and ONS-76 cells: increased stem cell population - Medullospheres from DAOY, UW228 and ONS-76 cells: increased stem cell population
Zanini C, Ercole E, Mandili G, Salaroli R, Poli A, Renna C, Papa V, Cenacchi G, Forni M. PLoS One. 2013 May 24;8(5):e63748.
MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell - MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell
Mathieu ME, Faucheux C, Saucourt C, Soulet F, Gauthereau X, Fedou S, Trouillas M, Theze N, Thiebaud P, Boeuf H. Development. 2013 Aug;140(16):3311-22.
A critical function of Mad2l2 in primordial germ cell development of mice. - A critical function of Mad2l2 in primordial germ cell development of mice.
Pirouz M, Pilarski S, Kessel M. PLoS Genet. 2013 Aug;9(8):e1003712.
Changes in sub-cellular localisation of trophoblast and inner cell mass specific - Changes in sub-cellular localisation of trophoblast and inner cell mass specific
Madeja ZE, Sosnowski J, Hryniewicz K, Warzych E, Pawlak P, Rozwadowska N, Plusa B, Lechniak D. BMC Dev Biol. 2013 Aug 13;13:32.
Effects of TET1 knockdown on gene expression and DNA methylation in porcine - Effects of TET1 knockdown on gene expression and DNA methylation in porcine
Fan A, Ma K, An X, Ding Y, An P, Song G, Tang L, Zhang S, Zhang P, Tan W, Tang B, Zhang X, Li Z. Reproduction. 2013 Oct 23;146(6):569-79.
Activation of pluripotency-associated genes in mouse embryonic fibroblasts by - Activation of pluripotency-associated genes in mouse embryonic fibroblasts by
Tavernier G, Wolfrum K, Demeester J, De Smedt SC, Adjaye J, Rejman J. Biomaterials. 2012 Jan;33(2):412-7.