POU5F1

Gene Symbol POU5F1
Entrez Gene 5460
Alt Symbol OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4
Species Human
Gene Type protein-coding
Description POU class 5 homeobox 1
Other Description POU domain transcription factor OCT4|POU domain, class 5, transcription factor 1|POU-type homeodomain-containing DNA-binding protein|octamer-binding protein 3|octamer-binding protein 4|octamer-binding transcription factor 3
Swissprots A6NLL8 Q5STF4 Q15167 Q01860 D2IYK4 A6NCS1 Q5STF3 Q15168 P31359 Q16422
Accessions ACZ95699 ACZ95700 AGG40970 AGG40971 AGG40974 BAB63311 BAC54946 BAE78630 BAF31281 EAX03366 EAX03367 EAX03368 Q01860 AA205411 AI811039 AM851115 CAO99189 AM851116 CAO99190 AM851117 CAO99191 AM851118 CAO99192 AM851119 CAO99193 AM851120 CAO99194 AM851121 CAO99195 AM851122 CAO99196 AM851123 CAO99197 AM851124 CAO99198 AM851125 CAO99199 AM851126 CAO99200 BC020712 BC069246 BC117435 AAI17436 BC117437 AAI17438 BQ278003 CD247724 DQ486514 DQ486515 DQ486516 DQ486517 EU518650 ACD14148 FM177951 CAQ76268 FM177952 CAQ76269 FM177953 CAQ76270 FM177954 CAQ76271 FM177955 CAQ76272 FM177956 CAQ76273 FM177957 CAQ76274 FM177958 CAQ76275 FM177959 CAQ76276 FM177960 CAQ76277 FM177961 CAQ76278 FM177962 CAQ76279 FM177963 CAQ76280 FM177964 CAQ76281 FM177965 CAQ76282 FM177968 CAQ76285 GU480808 ADE48539 GU480809 ADE48540 GU480810 GU480811 ADE48541 GU480812 ADE48542 GU480813 ADE48543 GU480815 ADE48544 GU480816 ADE48545 GU480817 ADE48546 GU480818 ADE48547 GU480819 ADE48548 GU480820 ADE48549 GU480821 ADE48550 GU480822 ADE48551 GU480823 ADE48552 GU480824 ADE48553 GU480825 ADE48554 GU480826 ADE48555 GU480827 ADE48556 GU480828 ADE48557 GU480829 ADE48558 GU480830 ADE48559 HQ122658 ADW77313 HQ122659 HQ122660 ADW77314 HQ122661 ADW77315 HQ122662 ADW77316 HQ122663 ADW77317 HQ122664 ADW77318 HQ122665 ADW77319 HQ122666 HQ122667 ADW77320 HQ122668 ADW77321 HQ122669 ADW77322 HQ122670 ADW77323 HQ122671 HQ122672 ADW77324 HQ122673 ADW77325 HQ122674 ADW77326 HQ122675 ADW77327 HQ258221 ADR82975 HQ258573 ADR83327 S81255 AAB35990 Z11898 CAA77951 Z11899 CAA77952 NM_001173531 NP_001167002 NM_001285986 NP_001272915 NM_001285987 NP_001272916 NM_002701 NP_002692 NM_203289 NP_976034
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. {ECO:0000269|PubMed:18035408}.
Subcellular Location Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern.
Tissue Specificity Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. {ECO:0000269|PubMed:1408763, ECO:0000269|PubMed:7908264}.
Top Pathways Signaling pathways regulating pluripotency of stem cells

Oct-4A (C30A3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 5177 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C30A3
Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa cells (right) using Oct-4A (C30A3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Oct-4A (C30A3) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 5263 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C30A3
Confocal immunofluorescent analysis of NTERA-2 (left) and HeLa cells (right) using Oct-4A (C30A3) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red).

Oct-4A (C30A3) Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 4439 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C30A3
Confocal immunofluorescent analysis of NTERA-2 cl.D1 (left) and HeLa cells (right) using Oct-4A (C30A3) Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green).

Oct-4 (V241) Antibody - 2788 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NCCIT and F9 cells using Oct-4 (V241) Antibody.

Oct-4 Antibody - 2750 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of NCCIT and NTERA2 cell extracts using Oct-4 Antibody.

Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) - 5677 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 immunoprecipitations)
Host Rabbit
Clonality Monoclonal
Clone C30A3C1
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCCIT cells and 10 µl of either Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or Nanog (D73G4) XP ® Rabbit mAb (ChIP Formulated) #5232, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5ng enriched ChIP DNA using NEBNext ® Ultra™ II DNA Library Prep Kit for Illumina ® , and sequenced on the Illumina NextSeq. Oct-4 and Nanog are known to associate with each other on chromatin. The figure shows binding of both Oct-4 and Nanog across POU5F1/Oct-4, a known target gene of both Oct-4 and Nanog (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Oct-4A (C52G3) Rabbit mAb - 2890 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C52G3
Western blot analysis of extracts from NCCIT and NTERA2 cells using Oct-4A (C52G3) Rabbit mAb.

Oct-4A (C30A3) Rabbit mAb - 2840 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C30A3
Western blot analysis of extracts from NTERA2 and mouse embryonic stem cells (mESCs) using Oct-4A (C30A3) Rabbit mAb.

Oct-4 (9B7) Mouse mAb - 4286 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 9B7
Western blot analysis of extracts from NTERA and F9 cell lines using Oct-4 (9B7) Mouse mAb.

Oct-3/4 (A-9) - sc-365509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Oct-3/4 (A-9): sc-365509. Western blot analysis of Oct-3/4 expression in F9 whole cell lysate.

Oct-3/4 (C-10) - sc-5279 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Oct-3/4 (C-10): sc-5279. Indirect, intracellular FCM analysis of fixed and permeabilized F9 cells stained with Oct-3/4 (C-10), followed by PE-conjugated goat anti-mouse IgG: sc-3738. Black line histogram represents the isotype control, normal mouse IgG<sub>2b</sub>: sc-3879.

Oct-3/4 (F-7) - sc-514295 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Oct-3/4 (F-7): sc-514295. Western blot analysis of Oct-3/4 expression in NTERA-2 cl.D1 (A), HeLa (B), Hep G2 (C) and HEK293 (D) whole cell lysates.

Oct-3/4 (C-20) - sc-8629 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Oct-3/4 (C-20): sc-8629. Immunoperoxidase staining of formalin-fixed, paraffin-embedded testicular pre-malignant carcinoma in situ showing nuclear staining. Kindly provided by Dr. Rajpert-De Meyts at University Hospital of Copenhagen, Denmark.

Oct-3/4 (H-134) - sc-9081 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Immunofluorescence staining of formalin-fixed human embryonic stem cells showing nuclear staining of Oct-3/4 (pink), cytoplasmic staining of L-Alkaline phosphatase (green) and blue counterstain. Antibodies tested include Oct-3/4 (H-134): sc-9081 and L-Alkaline phosphatase (TRA-2-39): sc-21708. Kindly provided by Dr. Nick Strelchenko at Reproductive Genetics Institute, Chicago.

Oct-3/4 (H-65) - sc-25401 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Oct-3/4 (H-65): sc-25401. Western blot analysis of human recombinant Oct-3/4 fusion protein.

Oct-3/4 (1) - sc-101534 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Oct-3/4 (1): sc-101534. Western blot analysis of human recombinant Oct-3/4.

Oct-3/4 (N-19) - sc-8628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Oct-3/4 (N-19): sc-8628. Western blot analysis of Oct-3/4 expression in F9 whole cell lysate.

Oct-3/4 (N-20) - sc-8630 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Oct4 antibody - ab18976 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab18976 at 1/100 dilution staining Oct-4 in mouse brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% BSA for 1 hour at 25°C and then incubated with ab18976 for 12 hours at 4°C. A Cy3 conjugated donkey polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody.See Abreview

Anti-Oct4 antibody - ab27985 from Abcam

Prices $400.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab27985 recognizes Oct4 in human embryonic stem cells. The upper panel shows DAPI staining while the lower panel shows ab27985 staining.See Abreview

Anti-Oct4 antibody - ab134218 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
ICC/IF image of ab134218 stained mES cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134218, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1:250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-Oct4 antibody - ab83498 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Oct4 antibody (ab83498) at 1/500 dilutionLane 1 : Mouse kidney tissue lysateLane 2 : Mouse kidney tissue lysate, pre-incubated with immunizing peptide

Anti-Oct4 antibody - ab125949 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Oct4 antibody (ab125949) at 1 µg/mlLane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell LysateLane 2 : mESC Wild Type LysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Oct4 antibody - ab53028 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Standard Curve for Oct4; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal to Oct4 (ab77259) at 5 ug/ml and Detector Antibody Rabbit polyclonal to Oct4 (ab53028) at 0.5 ug/ml.

Anti-Oct4 antibody - ab80700 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Oct4 antibody (ab80700) at 1 µg/mlLane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
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