Anti-Oct4 antibody
| Name | Anti-Oct4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab27985 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human Oct4 aa 300 to the C-terminus (C terminal) |
| Description | Goat Polyclonal |
| Gene | POU5F1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab27985 recognizes Oct4 in human embryonic stem cells. The upper panel shows DAPI staining while the lower panel shows ab27985 staining.See Abreview
ab27985 recognizes Oct4 in mouse embryonic stem cells. DAPI staining is shown in the top panel while ab27985 staining is shown in the lower panel.See Abreview
All lanes : Anti-Oct4 antibody (ab27985) at 1 µg/mlLane 1 : Human Embryonic Stem Cell LysateLane 2 : Mouse Embryonic Stem Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat IgG secondary antibody (ab28445) at 1/10000 dilution
ab27985 staining Oct4 in human embryonic carcinoma cells by Flow Cytometery. Cells were fixed in paraformaldehyde and permeabilized in saponin buffer. The primary antibody was diluted 1:500 (PBS + 10% BSA) and incubated with sample for 1 hour at 4°C. A FITC conjugated Donkey polyclonal to goat IgG, diluted 1:200 was used as secondary. The red histogram is secondary only control whilst green and blue repersents Oct4 expression in untreated and partially differentiated with 10nM Trans-Retinoic acid for 14 days. See Abreview
Product References
Germline transmission of an embryonic stem cell line derived from BALB/c cataract - Germline transmission of an embryonic stem cell line derived from BALB/c cataract
Peng X, Liu T, Shi C, Zhang L, Wang Y, Zhao W, Jiang L, Wu M, Zhang Y, Qian Q. PLoS One. 2014 Mar 4;9(3):e90707.
Partial somatic to stem cell transformations induced by cell-permeable - Partial somatic to stem cell transformations induced by cell-permeable
Lim J, Kim J, Kang J, Jo D. Sci Rep. 2014 Mar 12;4:4361.
RLIM is dispensable for X-chromosome inactivation in the mouse embryonic - RLIM is dispensable for X-chromosome inactivation in the mouse embryonic
Shin J, Wallingford MC, Gallant J, Marcho C, Jiao B, Byron M, Bossenz M, Lawrence JB, Jones SN, Mager J, Bach I. Nature. 2014 Jul 3;511(7507):86-9.
Patient-specific naturally gene-reverted induced pluripotent stem cells in - Patient-specific naturally gene-reverted induced pluripotent stem cells in
Tolar J, McGrath JA, Xia L, Riddle MJ, Lees CJ, Eide C, Keene DR, Liu L, Osborn MJ, Lund TC, Blazar BR, Wagner JE. J Invest Dermatol. 2014 May;134(5):1246-54.
Changes in sub-cellular localisation of trophoblast and inner cell mass specific - Changes in sub-cellular localisation of trophoblast and inner cell mass specific
Madeja ZE, Sosnowski J, Hryniewicz K, Warzych E, Pawlak P, Rozwadowska N, Plusa B, Lechniak D. BMC Dev Biol. 2013 Aug 13;13:32.
Effects of combined epidermal growth factor, brain-derived neurotrophic factor - Effects of combined epidermal growth factor, brain-derived neurotrophic factor
Yu Y, Yan J, Li M, Yan L, Zhao Y, Lian Y, Li R, Liu P, Qiao J. Hum Reprod. 2012 Jul;27(7):2146-59.
The effect of vitronectin on the differentiation of embryonic stem cells in a 3D - The effect of vitronectin on the differentiation of embryonic stem cells in a 3D
Heydarkhan-Hagvall S, Gluck JM, Delman C, Jung M, Ehsani N, Full S, Shemin RJ. Biomaterials. 2012 Mar;33(7):2032-40.
Role of tumor associated fibroblasts in human liver regeneration, cirrhosis, and - Role of tumor associated fibroblasts in human liver regeneration, cirrhosis, and
Cesselli D, Beltrami AP, Poz A, Marzinotto S, Comisso E, Bergamin N, Bourkoula E, Pucer A, Puppato E, Toffoletto B, Sorrentino M, Baccarani U, Avellini C, Beltrami CA. Int J Hepatol. 2011;2011:120925.
DA neurons derived from hES cells that express HLA-G1 are capable of - DA neurons derived from hES cells that express HLA-G1 are capable of
Zhu Y, Zhao H, Wang L, Zhao S, Jiang F, Li L, Yao Y. Brain Res. 2012 Feb 9;1437:134-42.
Hematopoietic differentiation of induced pluripotent stem cells from patients - Hematopoietic differentiation of induced pluripotent stem cells from patients
Tolar J, Park IH, Xia L, Lees CJ, Peacock B, Webber B, McElmurry RT, Eide CR, Orchard PJ, Kyba M, Osborn MJ, Lund TC, Wagner JE, Daley GQ, Blazar BR. Blood. 2011 Jan 20;117(3):839-47.