Anti-OLFM4 antibody
| Name | Anti-OLFM4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab85046 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human OLFM4 |
| Description | Rabbit Polyclonal |
| Gene | OLFM4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab85046 stained T84 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85046 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-OLFM4 antibody (ab85046) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of OLFM4 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85046, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
TIGAR is required for efficient intestinal regeneration and tumorigenesis. - TIGAR is required for efficient intestinal regeneration and tumorigenesis.
Cheung EC, Athineos D, Lee P, Ridgway RA, Lambie W, Nixon C, Strathdee D, Blyth K, Sansom OJ, Vousden KH. Dev Cell. 2013 Jun 10;25(5):463-77.
Cell lineage identification and stem cell culture in a porcine model for the - Cell lineage identification and stem cell culture in a porcine model for the
Gonzalez LM, Williamson I, Piedrahita JA, Blikslager AT, Magness ST. PLoS One. 2013 Jun 28;8(6):e66465.
The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent - The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent
Munoz J, Stange DE, Schepers AG, van de Wetering M, Koo BK, Itzkovitz S, Volckmann R, Kung KS, Koster J, Radulescu S, Myant K, Versteeg R, Sansom OJ, van Es JH, Barker N, van Oudenaarden A, Mohammed S, Heck AJ, Clevers H. EMBO J. 2012 Jun 12;31(14):3079-91.