OLFM4

Gene Symbol OLFM4
Entrez Gene 10562
Alt Symbol GC1, GW112, OLM4, OlfD, UNQ362, bA209J19.1, hGC-1, hOLfD
Species Human
Gene Type protein-coding
Description olfactomedin 4
Other Description G-CSF-stimulated clone 1 protein|antiapoptotic protein GW112|olfactomedin-4
Swissprots Q86T22 Q5VWG0 O95362 Q6UX06
Accessions EAW52052 EAW52053 Q6UX06 AF097021 AAC72970 AI560739 AK000683 AK300947 BAG62576 AY358567 AAQ88930 BC047740 AAH47740 BC117329 AAI17330 DA928098 HQ258281 ADR83035 NM_006418 NP_006409
Function May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. {ECO:0000269|PubMed:16566923, ECO:0000269|PubMed:17270022, ECO:0000269|PubMed:20724538}.
Subcellular Location Secreted, extracellular space. Mitochondrion. Note=Subcellular location is not clearly defined: has been shown to be secreted (PubMed:16566923), but also in the mitochondrion (PubMed:15059901 and PubMed:20724538), cytoplasm and plasma membrane (PubMed:20724538) and in the nucleus (PubMed:15059901). {ECO:0000269|PubMed:15059901, ECO:0000269|PubMed:16566923, ECO:0000269|PubMed:20724538}.
Tissue Specificity Expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues. {ECO:0000269|PubMed:11867215, ECO:0000269|PubMed:16566923, ECO:0000269|PubMed:17270022, ECO:0000269|PubMed:20724538}.

OLFM4 (D1E4M) XP ® Rabbit mAb - 14369 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1E4M
Western blot analysis of extracts from human small intestine, colon, and prostate, and two human cancer cell lines (OV-90 and MCF7), using OLFM4 (D1E4M) XP ® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). As expected, OLFM4 protein expression is not detected in MCF7 cells.

GC-1 (D-20) - sc-84274 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

GC-1 (N-20) - sc-84275 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
GC-1 (N-20): sc-84275. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing membrane and cytoplasmic staining of glandular cells.

GC-1 (S-18) - sc-84276 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
GC-1 (S-18): sc-84276. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing membrane and cytoplasmic staining of glandular cells.

Anti-OLFM4 antibody - ab85046 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab85046 stained T84 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85046 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-OLFM4 antibody - ab151529 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-OLFM4 antibody (ab151529) at 1 µg/mlLane 1 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-OLFM4 antibody - ab55937 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 antibody - ab195430 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 antibody - ab96280 from Abcam

Prices $382.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-OLFM4 antibody (ab96280) at 2 µg/mlLane 1 : Human liver lysateLane 2 : Human liver lysate with immunizing peptide

Anti-OLFM4 antibody [EPR9504(2)] - ab185221 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9504(2)
All lanes : Anti-OLFM4 antibody [EPR9504(2)] (ab185221) at 1/10000 dilutionLane 1 : LnCaP cell lysateLane 2 : PC3 cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-OLFM4 / Olfactomedin 4 Antibody (aa400-450) IHC-plus™ - LS-B2055 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-OLFM4 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2055 concentration 5 ug/ml.

Anti-OLFM4 / Olfactomedin 4 Antibody (Internal) IHC-plus™ - LS-A8454 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-OLFM4 antibody LS-A8454 IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-OLFM4 / Olfactomedin 4 Antibody (Internal) IHC-plus™ - LS-A9926 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-OLFM4 antibody LS-A9926 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-OLFM4 / Olfactomedin 4 Antibody (Internal) IHC-plus™ - LS-A8455 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-OLFM4 antibody LS-A8455 IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-OLFM4 / Olfactomedin 4 Antibody (N-Terminus) - LS-C47070 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 / Olfactomedin 4 Antibody (aa407-510, Biotin) - LS-C299032 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 / Olfactomedin 4 Antibody (aa238-500, Biotin) - LS-C299033 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 / Olfactomedin 4 Antibody (aa405-511, Biotin) - LS-C300762 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 / Olfactomedin 4 Antibody (aa238-500, FITC) - LS-C301534 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 / Olfactomedin 4 Antibody (aa407-510, FITC) - LS-C301536 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 / Olfactomedin 4 Antibody (aa405-511, FITC) - LS-C303685 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OLFM4 / Olfactomedin 4 Antibody (C-Terminus) - LS-C81617 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.

Anti-OLFM4 / Olfactomedin 4 Antibody (aa407-510) - LS-C295710 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant OLFM4 / Olfactomedin 4.

Anti-OLFM4 / Olfactomedin 4 Antibody (aa405-511) - LS-C295713 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant OLFM4 / Olfactomedin 4.

Anti-OLFM4 / Olfactomedin 4 Antibody (aa238-500) - LS-C295714 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant OLFM4 / Olfactomedin 4.
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