All lanes : Anti-OLFM4 antibody [EPR9504(2)] (ab185221) at 1/10000 dilutionLane 1 : LnCaP cell lysateLane 2 : PC3 cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab185221 at 1/50 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.