Anti-Olig2 antibody
| Name | Anti-Olig2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56643 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein: DSDASLVSSR PSSPEPDDLF LPARSKGSSG SAFTGGTVSS STPSDCPPEL SAELRGAMGS AGAHPGDKLG GSGFKSS, corresponding to amino acids 2-78 of Human Olig2 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | OLIG2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Standard Curve for Olig2; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal to Olig2 (ab56643) at 5 ug/ml and Detector Antibody Rabbit polyclonal to Olig2 (ab77953) at 0.5 ug/ml.
Western blot against tagged recombinant protein immunogen using ab56643 Olig2 antibody at 1ug/ml.
IHC image of ab56643 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56643, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.