![All lanes : Anti-Olig2 antibody [1G11] (ab110031) at 1/500 dilutionLane 1 : HEK 293 cell lysateLane 2 : Olig2-hIgGFc transfected HEK 293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3768_Olig2-Primary-antibodies-ab110031-1.JPG)
All lanes : Anti-Olig2 antibody [1G11] (ab110031) at 1/500 dilutionLane 1 : HEK 293 cell lysateLane 2 : Olig2-hIgGFc transfected HEK 293 cell lysate
Immunohistochemical analysis of Olig2 in paraffin embedded muscle tissue, using ab110031 with DAB staining.
Immunofluorescence analysis of Olig2 in U251 cells, using a 1/200 dilution of ab110031 (green). Blue: DRAQ5 fluorescent DNA dye.
ELISA results for ab110031.
![Overlay histogram showing SH-SY5Y cells stained with ab110031 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110031, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/3772_Olig2-Primary-antibodies-ab110031-5.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab110031 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110031, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.