All lanes : Anti-Optineurin antibody (ab151240) at 1/2000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : Hep G2 whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Optineurin with ab151240 at 1/500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling Optineurin with ab151240 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.