Anti-Optineurin antibody
| Name | Anti-Optineurin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23666 |
| Prices | $382.00 |
| Sizes | 200 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Bovine, Pig, Monkey, Monkey |
| Antigen | Synthetic peptide: GEVLPDIDTLQIHVMDC , corresponding to amino acids 575-591 of Human Optineurin |
| Description | Rabbit Polyclonal |
| Gene | OPTN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab23666 staining Optineurin in HeLa cells and Retinal Ganglion Cell line 5 cells (RGC-5) by Immunocytochemistry/ Immunofluorescence.(A) HeLa cells were pulsed with Alexa-546® conjugated transferrin for 30 minutes, chased with unlabeled holo-transferrin for 20 minutes and stained for endogenous Optineurin.(B) RGC-5 cells were stained for endogenous Optineurin.
Anti-Optineurin antibody (ab23666) at 1 µg + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab23666 staining Optineurin in Mouse cultured primary hippocampal neurons by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in 0.25% Triton in PBS prior to blocking in 5% Donkey serum for 30 minutes at 22°C. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at 22°C. The secondary antibody was Cy3®-conjugated Donkey anti-Rabbit polyclonal, diluted 1/500.See Abreview
Product References
Caspase-11 activation requires lysis of pathogen-containing vacuoles by - Caspase-11 activation requires lysis of pathogen-containing vacuoles by
Meunier E, Dick MS, Dreier RF, Schurmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P. Nature. 2014 May 15;509(7500):366-70.
E3-14.7K is recruited to TNF-receptor 1 and blocks TNF cytolysis independent from - E3-14.7K is recruited to TNF-receptor 1 and blocks TNF cytolysis independent from
Klingseisen L, Ehrenschwender M, Heigl U, Wajant H, Hehlgans T, Schutze S, Schneider-Brachert W. PLoS One. 2012;7(6):e38348.
Optineurin is required for CYLD-dependent inhibition of TNFalpha-induced - Optineurin is required for CYLD-dependent inhibition of TNFalpha-induced
Nagabhushana A, Bansal M, Swarup G. PLoS One. 2011 Mar 7;6(3):e17477.
Optineurin negatively regulates the induction of IFNbeta in response to RNA virus - Optineurin negatively regulates the induction of IFNbeta in response to RNA virus
Mankouri J, Fragkoudis R, Richards KH, Wetherill LF, Harris M, Kohl A, Elliott RM, Macdonald A. PLoS Pathog. 2010 Feb 19;6(2):e1000778.
Regulation of endocytic trafficking of transferrin receptor by optineurin and its - Regulation of endocytic trafficking of transferrin receptor by optineurin and its
Nagabhushana A, Chalasani ML, Jain N, Radha V, Rangaraj N, Balasubramanian D, Swarup G. BMC Cell Biol. 2010 Jan 19;11:4.
NF-kappaB mediates tumor necrosis factor alpha-induced expression of optineurin, - NF-kappaB mediates tumor necrosis factor alpha-induced expression of optineurin,
Sudhakar C, Nagabhushana A, Jain N, Swarup G. PLoS One. 2009;4(4):e5114.