Phospho-Cyclin B1 (Ser116) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 13495
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser116 of human cyclin B1 protein
• Description: Rabbit Polyclonal
• Gene: CCNB1

Product images

Western blot analysis of HT-29 cell extracts, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Phospho-Cyclin B1 (Ser116) Antibody (upper), Cyclin B1 (D5C10) XP ® Rabbit mAb #12231 (middle), or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).

Western blot analysis of HT-29 cell extracts, untreated (AS) or synchronized in S-phase by double thymidine block (2 mM) followed by release into fresh medium for the indicated time, using Phospho-Cyclin B1 (Ser116) Antibody (upper), Cyclin B1 (D5C10) XP ® Rabbit mAb #12231 (middle), or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).

Western blot anaylsis of HeLa cell extracts, untreated (-) or treated with Nocodazole #2190 (10 nM, 24 hr; +) using Phospho-Cyclin B1 (Ser116) Antibody (upper), Cyclin B1 (D5C10) XP® Rabbit mAb #12231 (middle), or α-Actinin (D6F6) XP® rabbit mAb #6487 (lower). Membranes were mock treated (- CIP) or Calf Intestinal Alkaline Phosphatase treated (+ CIP) after transfer.