CCNB1

Gene Symbol CCNB1
Entrez Gene 891
Alt Symbol CCNB
Species Human
Gene Type protein-coding
Description cyclin B1
Other Description G2/mitotic-specific cyclin B1|G2/mitotic-specific cyclin-B1
Swissprots Q5TZP9 A8K066 P14635
Accessions AAD14154 AAD14818 AAP88038 CBX86318 EAW51306 P14635 AI972071 AK289431 BAF82120 AM851053 CAO99273 AY027761 AAK27417 AY027762 AAK27418 AY027763 AAK27419 AY027764 AAK27420 AY027765 AAK27421 AY027767 AAK27422 AY027768 AAK27423 AY027769 AAK27424 AY027770 AAK27425 BC006510 AAH06510 BI253686 BM979016 BT020128 AAV38930 DQ892975 ABM83901 M25753 NM_031966 NP_114172
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition. {ECO:0000269|PubMed:17495531, ECO:0000269|PubMed:17495533}.
Subcellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Top Pathways Cell cycle, p53 signaling pathway, Progesterone-mediated oocyte maturation, Oocyte meiosis

Cyclin B1 (V152) Mouse mAb (Alexa Fluor® 488 Conjugate) - 4112 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone V152
Flow cytometric analysis of untreated Jurkat cells, using Cyclin B1 (V152) (Alexa Fluor®488 Conjugate) Mouse mAb versus propidium iodide (DNA content).

Cyclin B1 (V152) Mouse mAb (Alexa Fluor ® 647 Conjugate) - 4118 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone V152
Two-color flow cytometric analysis of asynchronous Jurkat cells, using Cyclin B1 (V152) Alexa Fluor ® 647 Conjugate and Phospho-Histone H3 (Ser10) Alexa Fluor ® 488 Conjugate #9708. Cells represented in green are positive for cyclin B1 and phospho-histone H3, while cells represented in blue are positive for phospho-histone H3 and negative for cyclin B1. Both cell populations (green and blue) correspond to cells undergoing mitosis.

Phospho-Cyclin B1 (Ser116) Antibody - 13495 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of HT-29 cell extracts, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Phospho-Cyclin B1 (Ser116) Antibody (upper), Cyclin B1 (D5C10) XP ® Rabbit mAb #12231 (middle), or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).

Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb - 4133 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 9E3
Western blot analysis of extracts from HeLa cells, treated with hydroxyurea or nocodazole for 24 hours, using Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb (upper) or Cyclin B1 (U152) Mouse mAb #4135 (lower).

Phospho-Cyclin B1 (Ser147) Antibody - 4131 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 cells, using Phospho-Cyclin B1 (Ser147) Antibody (A) and Cyclin B1 (V152) Monoclonal Antibody (C). Treatment of the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer abolishes the phospho-cyclin B1 signal (B), but has no effect on the total cyclin B1 signal (D).

Cyclin B1 (D5C10) XP ® Rabbit mAb - 12231 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5C10
Western blot analysis of extracts from various cell lines using Cyclin B1 (D5C10) XP ® Rabbit mAb.

Cyclin B1 (V152) Mouse mAb - 4135 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (20 western blots)
Host Mouse
Clonality Monoclonal
Clone V152
Western blot analysis of extracts from HT29 cells, control (cont.) or nocodazole-treated (50 ng/ml) (M), using Phospho-cyclin B1 (Ser147) Antibody #4131 (upper), Cyclin B1 (V152) Mouse mAb (middle), or Cdc25C Antibody #9522 (lower).

Cyclin B1 Antibody - 4138 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Cyclin B1 Antibody.

cyclin B1 (G-11) - sc-166757 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cyclin B1 (G-11): sc-166757. Western blot analysis of cyclin B1 expression in K-562 (A) and Jurkat (B) nuclear extracts.

cyclin B1 (GNS1) - sc-245 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cyclin B1 (GNS1): sc-245. Western blot analysis of cyclin B1 expression in untreated (A) and phorbol ester-induced (B) K-562 nuclear extracts.

cyclin B1 (H-433) - sc-752 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
cyclin B1 (H-433): sc-752. Western blot analysis of cyclin B1 expression in untreated (A,C,E) and phorbol ester-induced (B,D,F) K-562 (A,B), Jurkat (C,D) and HeLa (E,F) nuclear extracts.

cyclin B1 (D-11) - sc-7393 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cyclin B1 (D-11) Alexa Fluor 647: sc-7393 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-24636.

cyclin B1 (H-20) - sc-594 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
cyclin B1 (H-20): sc-594. Western blot analysis of cyclin B1 expression in untreated (A,C,E) and phorbol ester-induced (B,D,F) K-562 (A,B), Jurkat (C,D) and HeLa (E,F) nuclear extracts.

cyclin B1 (M-20) - sc-595 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
cyclin B1 (M-20): sc-595. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

cyclin B1 (V152) - sc-53236 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cyclin B1 (V152): sc-53236. Western blot analysis of cyclin B1 expression in HeLa (A), PMA treated HeLa (B), K-562 (C), PMA treated K-562 (D), Jurkat (E) and PMA treated Jurkat (F) nuclear extracts.

cyclin B1 (V92.1) - sc-53238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cyclin B1 (V92.1): sc-53238. Western blot analysis of cyclin B1 expression in K-562 (A), PMA treated K-562 (B), Jurkat (C) and PMA induced Jurkat (D) nuclear extracts.

cyclin B1 (0.N.238) - sc-70898 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
cyclin B1 (0.N.238): sc-70898. Western blot analysis of cyclin B1 expression in non-transfected: sc-117752 (A) and mouse cyclin B1 transfected: sc-119543 (B) 293T whole cell lysates and K-562 nuclear extract (C).

p-cyclin B1 (Ser 147) - sc-293098 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-cyclin B1 (Ser 147): sc-293098. Western blot analysis of cyclin B1 phosphorylation expression in untreated (A) and anisomycin treated (B) MDA whole cell lysates.

p-cyclin B1 (Ser 128) - sc-130591 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-cyclin B1 (Ser 128): sc-130591. Western blot analysis of cyclin B1 phosphorylation in K-562 whole cell lysate.

Anti-Cyclin B1 (phospho S126) antibody - ab55184 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilutionLane 1 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins).Lane 2 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.

Anti-Cyclin B1 (phospho S126) antibody - ab3488 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilutionLane 1 : HeLa whole cell lysateLane 2 : H23 whole cell lysateLane 3 : Hepatocarcinoma (Hep3b) cell lysateLane 4 : Glioblastoma (T98G) cell lysate Lane 5 : B cell lymphoblast (Daudi) cell lysateLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Cyclin B1 (phospho S126) antibody [EPR3670] - ab133439 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3670
All lanes : Anti-Cyclin B1 (phospho S126) antibody [EPR3670] (ab133439) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Lysate of HeLa cells treated with Nocodazole Lysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Anti-Cyclin B1 (phospho S126) antibody [ilv-phos4.1] - ab97712 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone ilv-phos4.1

Anti-Cyclin B1 (phospho S147) antibody - ab60986 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab60986 at 1/50-1/100 dilution, with or without the immunising phosphopeptide.

Anti-Cyclin B1 (phospho S147) antibody - ab131502 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131502 at 1/100 dilution.
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