Anti-OS9 antibody
| Name | Anti-OS9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab19853 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human OS9 |
| Description | Rabbit Polyclonal |
| Gene | OS9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of OS9 staining in human hippocampus FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19853, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-OS9 antibody (ab19853) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 4 : Spleen (Mouse) Tissue LysateLane 5 : Liver (Mouse) Tissue Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab19853 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19853, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Reconstructed glycan profile for evaluation of operating status of the - Reconstructed glycan profile for evaluation of operating status of the
Iwamoto S, Isoyama M, Hirano M, Yamaya K, Ito Y, Matsuo I, Totani K. Glycobiology. 2013 Jan;23(1):121-31.
The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation. - The ERdj5-Sel1L complex facilitates cholera toxin retrotranslocation.
Williams JM, Inoue T, Banks L, Tsai B. Mol Biol Cell. 2013 Mar;24(6):785-95.
MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum - MHC class I molecules are preferentially ubiquitinated on endoplasmic reticulum
Burr ML, van den Boomen DJ, Bye H, Antrobus R, Wiertz EJ, Lehner PJ. Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14290-5. doi:
OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin - OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin
Christianson JC, Shaler TA, Tyler RE, Kopito RR. Nat Cell Biol. 2008 Mar;10(3):272-82.
SEL1L nucleates a protein complex required for dislocation of misfolded - SEL1L nucleates a protein complex required for dislocation of misfolded
Mueller B, Klemm EJ, Spooner E, Claessen JH, Ploegh HL. Proc Natl Acad Sci U S A. 2008 Aug 26;105(34):12325-30. doi: