E2F-1 (C-20): sc-193. Western blot analysis of BALB/3T3 cells transfected with either y-CRE (A) or y-CRE/E2F (B).
ChIP analysis of E2F transcription factor binding to the c-Myc promoter in NBS cells. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced with permission from Master, et al. 2001. Mol. Cell. Biol. 21: 6006-6016.
Extracts of 293 cells transiently transfected with plasmid containing the murine carboxylesterase promoter were subjected to ChIP analysis using the following E2F antibodies: E2F-1 (C-20) X: sc-193 X (A), E2F-2 (C-20) X: sc-633 X (B), E2F-3 (C-18) X: sc-878 X (C), E2F-4 (C-20) X: sc-866 X (D), E2F-5 (E-19) X: sc-999 X (E), E2F-6 (E-20) X: sc-8366 X (F). Controls: normal rabbit IgG: sc-2027 (G), no antibody (H), input DNA (I) and mock (J). Data kindly provided by J. L. Lavrrar and reproduced from J. Biol. Chem. 279: 46343-46349, 2004, with permission from the American Society for Biochemistry and Molecular Biology.
ChIP analysis of E2F transcription factor binding to the oriP (I.) and c-Myc (II.) promoters in Raji cells as a function of the cell cycle. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced from Master, et al., 2001. Mol. Cell. Biol. 21: 6006-6016.
E2F-1 (C-20): sc-193. Western blot analysis of E2F-1 expression in non-transfected: sc-117752 (A) and human E2F-1 transfected: sc-116659 (B) 293T whole cell lysates.
E2F-1 (C-20): sc-193. Western blot analysis of E2F-1 expression in non-transfected: sc-117752 (A) and human E2F-1 transfected: sc-116137 (B) 293T whole cell lysates.
E2F-1 (C-20): sc-193. Western blot analysis of E2F-1 expression in non-transfected: sc-117752 (A) and mouse E2F-1 transfected: sc-126755 (B) 293T whole cell lysates.
E2F-1 (C-20): sc-193. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
E2F-1 CRISPR/Cas9 KO Plasmid (h): sc-400123. Western blot analysis of human E2F-1 expression in Control CRISPR/Cas9 Plasmid transfected: sc-418922 (A) and E2F-1 CRISPR/Cas9 KO Plasmid transfected (B) 293T whole cell lysates. Blot probed with E2F-1 (C-20): sc-193. GAPDH (0411): sc-47724 used as specificity and loading control.