Anti-Osteopontin antibody [7C5H12]

• Supplier: Abcam
• Catalog: ab166709
• Prices: $394.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 7C5H12
• Applications: WB ELISA IHC-P ICC/IF
• Species Reactivities: Human, Chimpanzee
• Antigen: Purified recombinant fragment, corresponding to amino acids 167-314 of Human Osteopontin, expressed in E
• Description: Mouse Monoclonal
• Gene: SPP1
• Conjugate: Unconjugated

Product images

ab166709 staining Osteopontin in human colon (smooth muscle cells) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview

Anti-Osteopontin antibody [7C5H12] (ab166709) at 1/500 dilution + recombinant Human Osteopontin protein

All lanes : Anti-Osteopontin antibody [7C5H12] (ab166709) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : HEK293 cell lysate, transfected with recombinant Human Osteopontin fragment (amino acids 167-314)

Immunohistochemical analysis of paraffin embedded, DAB-stained Human prostate cancer tissue labeling Osteopontin using ab166709 at a 1/200 dilution.

Immunohistochemical analysis of paraffin embedded, DAB-stained Human endometrial cancer tissue, labeling Osteopontin using ab166709 at a 1/200 dilution.