SPP1

Gene Symbol SPP1
Entrez Gene 6696
Alt Symbol BNSP, BSPI, ETA-1, OPN
Species Human
Gene Type protein-coding
Description secreted phosphoprotein 1
Other Description SPP1/CALPHA1 fusion|early T-lymphocyte activation 1|nephropontin|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)|urinary stone protein|uropontin
Swissprots Q8NBK2 Q567T5 Q15683 B2RDA1 Q15682 Q4W597 Q15681 Q96IZ1 P10451
Accessions AAA86886 AAY41035 BAA03554 CAX69239 CBX54353 EAX06004 EAX06005 EAX06006 EAX06007 EAX06008 EAX06009 EAX06010 P10451 AA665210 AB209987 BAE45628 AB469789 BAH58215 AF052124 AAC28619 AK057738 AK075463 BAC11635 AK290090 BAF82779 AK290104 BAF82793 AK295491 BAH12087 AK296035 BAG58801 AK315461 BAG37848 AM392768 CAL37646 AM392898 CAL37776 AY956318 AAX59003 BC007016 AAH07016 BC017387 AAH17387 BC022844 AAH22844 BC093033 AAH93033 BF699765 BX648003 CB117856 D28759 BAA05949 D28760 BAA05950 D28761 BAA05951 DA419665 DQ839491 ABI63352 DQ846870 ABI63357 DQ846871 ABI63358 DQ892544 ABM83470 DQ895755 ABM86681 EU831940 ACE87381 EU832035 ACE86697 HQ874460 AEA49027 HQ874461 AEA49028 J04765 AAA59974 JF412666 AEA49030 JF412667 AEA49031 M83248 AAA17675 X13694 CAA31984 NM_000582 NP_000573 NM_001040058 NP_001035147 NM_001040060 NP_001035149 NM_001251829 NP_001238758 NM_001251830 NP_001238759
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Subcellular Location Secreted.
Tissue Specificity Bone. Found in plasma.
Top Pathways Focal adhesion, ECM-receptor interaction, Toll-like receptor signaling pathway, PI3K-Akt signaling pathway

OPN (P-18) - sc-10593 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
OPN (P-18): sc-10593. Western blot analysis of mouse recombinant OPN.

OPN (AKm2A1) - sc-21742 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
OPN (AKm2A1): sc-21742. Western blot analysis of mouse recombinant OPN.

OPN (K-20) - sc-10591 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
OPN (K-20): sc-10591. Western blot analysis of OPN expression in 293T whole cell lysate.

OPN (FL-314) - sc-20788 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
OPN (FL-314): sc-20788. Western blot analysis of OPN expression in CCD-1064Sk whole cell lysate.

OPN (LFMb-14) - sc-73631 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
OPN (LFMb-14): sc-73631. Immunoperoxidase staining of formalin fixed, paraffin-embedded tissue from mouse kidney tissue extract.

Anti-Osteopontin antibody - ab8448 from Abcam

Prices $400.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Osteopontin antibody (ab8448) at 1/1000 dilutionLane 2 : Human OsteopontinLane 3 : MMP-cleaved Human OsteopontinLysates/proteins at 0.25 µg per lane.SecondaryHRP-conjugated Goat anti-Rabbit IgG at 1/10000 dilution

Anti-Osteopontin antibody - ab63856 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Osteopontin antibody (ab63856) at 1 µg/mlLane 1 : Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376)Lane 2 : Brain (Rat) Tissue Lysate - normal tissueLane 3 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate (Human) Whole Cell LysateLane 4 : Brain (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-Osteopontin antibody - ab33046 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Osteopontin antibody (ab33046) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti Rabbit IgG at 1/50000 dilution

Anti-Osteopontin antibody - ab36125 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Osteopontin antibody (ab36125) at 0.3 µg/ml + Human Thymus lysate (RIPA buffer, 30µg total protein per lane).

Anti-Osteopontin antibody - ab14175 from Abcam

Prices $373.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-Osteopontin antibody - ab95090 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Osteopontin antibody (ab95090) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Osteopontin antibody - ab53772 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Osteopontin antibody (ab53772) at 1/500 dilutionLane 1 : Extracts from LOVO cellsLane 2 : Extracts from LOVO cells, plus immunising peptide

Anti-Osteopontin antibody - ab58231 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Osteopontin antibody - ab53364 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Osteopontin antibody - ab14176 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-Osteopontin antibody [14C08] - ab115871 from Abcam

Prices $368.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone 14C08

Anti-Osteopontin antibody [1F11] - ab72526 from Abcam

Prices $372.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone 1F11

Anti-Osteopontin antibody [2H9] - ab72525 from Abcam

Prices $372.00
Sizes 250 µg
Host Mouse
Clonality Monoclonal
Clone 2H9

Anti-Osteopontin antibody [53] - ab69498 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 53
All lanes : Anti-Osteopontin antibody [53] (ab69498) at 5 µg/mlLane 1 : MDA-MB-435 cell lysate at 40 µgLane 2 : Conditioned medium from MDA-MB-435 cells containing secreted Osteopontin at 3.8 µgLane 3 : Conditioned medium from MDA-MB-435 cells containing recombinant protein at 0.05 µgSecondaryHRP conjugated goat anti-mousedeveloped using the ECL technique

Anti-Osteopontin antibody [7C5H12] - ab166709 from Abcam

Prices $394.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7C5H12
ab166709 staining Osteopontin in human colon (smooth muscle cells) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview

Anti-Osteopontin antibody [EPR3688] - ab91655 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3688
All lanes : Anti-Osteopontin antibody [EPR3688] (ab91655) at 1/1000 dilutionLane 1 : MOLT-4 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit at 1/2000 dilution

Anti-Osteopontin antibody [EPR3688] (Alexa Fluor® 488) - ab196445 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3688
Overlay histogram showing U2OS cells stained with ab196445 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196445, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Osteopontin antibody [EPR3688] (Alexa Fluor® 647) - ab196177 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3688
Overlay histogram showing U2OS cells stained with ab196177 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196177, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

Anti-Osteopontin antibody [EPR3688] (HRP) - ab195541 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3688
IHC image of Osteopontin staining in a section of formalin-fixed paraffin-embedded normal human pancreatic tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195541 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Osteopontin antibody, prediluted - ab15489 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15489 staining Osteopontin in human breast carcinoma by Immunohistochemistry (FFPE-sections).
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