IHC-P image of Otx2 antibody (ab114138) on paraffin-embedded mouse embryo sections. The image on the left is of a WT embryo, whilst on the right is an embryo where Otx2 has been replaced with human Otx1 (hOtx1Otx2/Otx2). The sections underwent heat mediated antigen retrieval using Citrate Buffer, primary antibody was used at 1:500 dilution and Biocare Mach4 Kit was used to detect the primary antibody.
IHC-P image of Otx2 staining on Rat E12 embryo using ab114138 (1:1500). The sections were deparffinized and subjected to heat mediated antigen retrieval using citric acid. The section were then blocked using 1% BSA for 10 mins at 21°C. ab114138 was diluted 1:1500 using TBS/BSA/Azide and incubated for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1:250).
IHC-P image of Otx2 staining on mouse E14 embryo using ab114138 (1:1000). The sections were deparffinized and subjected to heat mediated antigen retrieval using citric acid. The section were then blocked using 1% BSA for 10 mins at 21°C. ab114138 was diluted 1:1000 using TBS/BSA/Azide and incubated for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to Biotin (1:250).
All lanes : Anti-Otx2 antibody (ab114138) at 1 µg/mlLane 1 : OTX1 (Human) - Recombinant ProteinLane 2 : OTX2 Transient Overexpression LysateLysates/proteins at 0.1 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Otx2 antibody (ab114138) at 1 µg/mlLane 1 : E10 Mouse Embryo Brain Tissue LysateLane 2 : E14 Mouse Embryo Brain Tissue LysateLane 3 : E16 Ms Embryo Brain Tissue LysateLane 4 : Eye (Mouse) Whole Cell Lysate - normal tissue (ab4029)Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLane 6 : WERI (Human Retinoblastoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.