![Overlay histogram showing Y79 cells stained with ab130238 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130238, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in LoVo cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5014_ab130238-4-ab130238FC.jpg)
Overlay histogram showing Y79 cells stained with ab130238 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130238, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in LoVo cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Otx2 antibody [1H12C4B5] (ab130238) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : NTERA 2 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5015_Otx2-Primary-antibodies-ab130238-1.JPG)
All lanes : Anti-Otx2 antibody [1H12C4B5] (ab130238) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : NTERA 2 cell lysate
![Anti-Otx2 antibody [1H12C4B5] (ab130238) at 1/500 dilution + Human Otx2 recombinant protein (aa: 40-297](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5016_Otx2-Primary-antibodies-ab130238-2.JPG)
Anti-Otx2 antibody [1H12C4B5] (ab130238) at 1/500 dilution + Human Otx2 recombinant protein (aa: 40-297
Analysis of ab130238 by ELISA.