Western blot analysis of immunoprecipitation pellet from SKBR3 cell lysate immunoprecipitated using ab185960 at 1/50 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
All lanes : Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/10000 dilutionLane 1 : SKBR3 cell lysateLane 2 : A375 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
All lanes : Anti-OVCA1 antibody [EPR15342] - C-terminal (ab185960) at 1/1000 dilutionLane 1 : SK OV 3 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution