All lanes : Anti-P cadherin antibody [6A9] (ab19350) at 1/500 dilutionLane 1 : 293T cell lysateLane 2 : A431 cell lysateLane 3 : Mouse brain tissue lysateLysates/proteins at 25 µg per lane.SecondaryHRP-conjugated anti-mouse IgG
Immunocytochemistry/Immunofluorescent analysis of A2058 cells labelling P Cadherin (green) with ab19350. Cells were grown on chamber slides and fixed with formaldehyde. Cells were incubated without (control, right) or with the primary antibody at a 1/20 dilution overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).
Immunocytochemistry/Immunofluorescent analysis of BEAS-2B cells labelling P Cadherin (green) with ab19350. Cells were grown on chamber slides and fixed with formaldehyde. Cells were incubated without (control, right) or with the primary antibody at a 1/20 dilution overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).
Immunocytochemistry/Immunofluorescent analysis of HeLa cells labelling P Cadherin (green) with ab19350. Cells were grown on chamber slides and fixed with formaldehyde. Cells were incubated without (control, right) or with the primary antibody at a 1/20 dilution overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).