Overlay histogram showing HeLa cells stained with ab126625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126625, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-p150 CAF1 antibody [EPR5576(2)] (ab126625) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysate, treated with hydroxyoureaLane 3 : HeLa cell line lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/5251_p150-CAF1-Primary-antibodies-ab126625-1.jpg)
All lanes : Anti-p150 CAF1 antibody [EPR5576(2)] (ab126625) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysate, treated with hydroxyoureaLane 3 : HeLa cell line lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution
ab126625, at 1/100, staining p150 CAF1 in formalin fixed, paraffin embedded Human colon tissue by Immunohistochemistry.
ab126625, at 1/100, staining p150 CAF1 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry.