CHAF1A

Gene Symbol CHAF1A
Entrez Gene 10036
Alt Symbol CAF-1, CAF1, CAF1B, CAF1P150, P150
Species Human
Gene Type protein-coding
Description chromatin assembly factor 1, subunit A (p150)
Other Description CAF-1 subunit A|CAF-I 150 kDa subunit|CAF-I p150|CTB-50L17.7|chromatin assembly factor 1 subunit A|chromatin assembly factor I (150 kDa)|chromatin assembly factor I p150 subunit|hp150
Swissprots Q7Z7K3 Q6NXG5 Q13111 Q9UJY8
Accessions AAF04291 EAW69224 EAW69225 EAW69226 Q13111 AA576757 BC005813 BC011560 BC041606 BC052620 AAH52620 BC067093 AAH67093 BE796132 BG775588 BM826511 BQ889365 H98924 HG510268 U20979 AAA76736 XM_011527605 XP_011525907 XM_011527606 XP_011525908 XM_011527607 XP_011525909 XR_936135 NM_005483 NP_005474
Function Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). {ECO:0000250, ECO:0000269|PubMed:15327775}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10052459}. Note=DNA replication foci.

CHAF1A (D77D5) XP ® Rabbit mAb - 5480 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D77D5
Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cell lines using CHAF1A (D77D5) XP ® Rabbit mAb.

CAF-1 p150 (K-16) - sc-10203 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CAF-1 p150 (K-16): sc-10203. Western blot analysis of CAF-1 p150 expression in K-562 (A) and Jurkat (B) nuclear extracts.

CAF-1 p150 (D-1) - sc-133105 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p150 (D-1): sc-133105. Western blot analysis of CAF-1 p150 expression in K-562 (A) and Jurkat (B) nuclear extracts.

CAF-1 p150 (C-21) - sc-10205 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CAF-1 p150 (C-21): sc-10205. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with CAF-1 p150 (C-21), followed by FITC-conjugated donkey anti-goat IgG: sc-2024. Black line histogram represents the isotype control, normal goat IgG: sc-3887.

CAF-1 p150 (26) - sc-136500 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p150 (26): sc-136500. Western blot analysis of CAF-1 p150 expression in SW13 whole cell lysate.

CAF-1 p150 (C-3) - sc-137141 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p150 (C-3): sc-137141. Western blot analysis of CAF-1 p150 expression in K-562 (A) and Jurkat (B) nuclear extracts.

CAF-1 p150 (H-300) - sc-10772 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CAF-1 p150 (H-300): sc-10772. Western blot analysis of CAF-1 p150 expression in HeLa (A) and K-562 (B) nuclear extracts.

CAF-1 p150 (p150-1) - sc-56642 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p150 (p150-1): sc-56642. Western blot analysis of CAF-1 p150 expression in K-562 (A) and Jurkat (B) nuclear extracts.

CAF-1 p150 (p150-48) - sc-32742 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p150 (p150-48): sc-32742. Western blot analysis of CAF-1 p150 expression in HeLa (A) and K-562 (B) nuclear extracts.

CAF-1 p150 (SS 1 1-13) - sc-56643 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p150 (SS 1 1-13): sc-56643. Western blot analysis of CAF-1 p150 expression in HeLa (A) and K-562 (B) nuclear extracts.

CAF-1 p150 (C-5) - sc-133068 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p150 (C-5): sc-133068. Western blot analysis of CAF-1 p150 expression in K-562 (A) and Jurkat (B) nuclear extracts.

CAF-1 p150 (D-16) - sc-10206 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CAF-1 p150 (D-16): sc-10206. Western blot analysis of human recombinant CAF-1 p150.

Anti-p150 CAF1 antibody - ab196768 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p150 CAF1 antibody (ab196768) at 1/500 dilutionLane 1 : A562 cell extractLane 2 : K562 cell extract

Anti-p150 CAF1 antibody - ab101286 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab101286, at 1/250 dilution, staining p150 CAF1 in a formalin-fixed paraffin-embedded section of Human colonic lymphoid aggregate by Immunohistochemistry using DAB staining.

Anti-p150 CAF1 antibody [1SS-48] - ab30327 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1SS-48

Anti-p150 CAF1 antibody [EPR5576(2)] - ab126625 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5576(2)
Overlay histogram showing HeLa cells stained with ab126625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126625, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CHAF1A / CAF1 Antibody - LS-C125365 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-CHAF1A / CAF-1 Antibody (aa21-120, clone 10B2214, Azide-free) - LS-C183517 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10B2214

Anti-CHAF1A / CAF1 Antibody (clone 2168C3a) - LS-C184718 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2168C3a

Anti-CHAF1A / CAF1 Antibody (aa21-120) - LS-C306928 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CHAF1A / CAF1 Antibody (aa878-938) - LS-C288277 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CHAF1A / CAF1 Antibody (clone p150-48) - LS-C179637 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone p150-48

Anti-CHAF1A / CAF1 Antibody - LS-C332007 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using CHAF1A antibody.

Anti-CHAF1A / CAF1 Antibody (aa500-550) - LS-C286204 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colonic lymphoid aggregate. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:250.

Anti-CHAF1A / CAF1 Antibody (aa100-150) - LS-C288275 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
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